Nigro G, Luzi G, Fridell E, Ferrara M, Pisano P, Gattinara G C, Mezzaroma I, Söderlund M, Rasnoveanu D, Aiuti F
Department of Pediatrics, La Sapienza University, Rome, Italy.
AIDS. 1992 Jul;6(7):679-84.
Investigation of the prevalence and pathogenic role of parvovirus B19 infection in Italian and Rumanian children with AIDS, compared with age-matched HIV-negative children (controls) with various recurrent infections of unknown aetiology.
Detection of B19-specific immunoglobulin (Ig) M and IgG antibodies as the most indicative markers of past or current B19 infection.
B19 antibodies were detected by two enzyme immunoassays using synthetic peptide or recombinant protein, which corresponded to different B19 epitopes, as coating antigens.
B19 IgM and IgG were seen in 10 out of 20 (50%) Italian and in 20 out of 51 (39.2%) Rumanian children with AIDS, in contrast to none out of 17 Italian and one out of 22 Rumanian controls (P less than 0.001). In addition, two Italian controls (11.8%), two Rumanian children with AIDS (3.9%), and two Rumanian controls (9.1%) had B19 IgM alone. Specific IgG alone was detected in eight (40%) Italian and 14 (27.5%) Rumanian children with AIDS, and in seven (41.2%) Italian and four (10.2%) Rumanian controls.
While it is possible to attribute some B19 infections in Rumanian children to blood transfusion, the source was unknown for Italian children. However, in three of the Italian children who had B19 IgM and IgG persistently for 15-22 months, and in a 2-month-old Italian infant with B19 IgM and IgG, HIV might have activated a congenital or perinatally-acquired B19 infection.
调查意大利和罗马尼亚患艾滋病儿童中细小病毒B19感染的患病率及其致病作用，并与年龄匹配的、病因不明的反复感染的HIV阴性儿童（对照组）进行比较。
检测B19特异性免疫球蛋白（Ig）M和IgG抗体，作为既往或当前B19感染的最具指示性的标志物。
采用两种酶免疫测定法检测B19抗体，使用对应于不同B19表位的合成肽或重组蛋白作为包被抗原。
20名意大利患艾滋病儿童中有10名（50%）、51名罗马尼亚患艾滋病儿童中有20名（39.2%）检测到B19 IgM和IgG，相比之下，17名意大利对照组儿童中无一例、22名罗马尼亚对照组儿童中仅有1例检测到（P<0.001）。此外，2名意大利对照组儿童（11.8%）、2名罗马尼亚患艾滋病儿童（3.9%）和2名罗马尼亚对照组儿童（9.1%）仅检测到B19 IgM。仅检测到特异性IgG的情况为：8名（40%）意大利患艾滋病儿童、14名（27.5%）罗马尼亚患艾滋病儿童、7名（41.2%）意大利对照组儿童和4名（10.2%）罗马尼亚对照组儿童。
虽然罗马尼亚儿童的一些B19感染可能归因于输血，但意大利儿童的感染源不明。然而，在3名持续15 - 22个月检测到B19 IgM和IgG的意大利儿童以及1名2个月大检测到B感染IgM和IgG的意大利婴儿中，HIV可能激活了先天性或围生期获得的B19感染。
