甘薯天蛾（Agrius convolvuli）蛹滞育终止早期大脑中细胞色素c氧化酶亚基1基因的表达

Expression of cytochrome c oxidase subunit 1 gene in the brain at an early stage in the termination of pupal diapause in the sweet potato hornworm, Agrius convolvuli.

作者信息

Uno Tomohide, Nakasuji Ai, Shimoda Masami, Aizono Yasuo

机构信息

Laboratory of Biological Chemistry, Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Nada-Ku, Kobe, Hyogo 657-8501, Japan.

出版信息

J Insect Physiol. 2004 Jan;50(1):35-42. doi: 10.1016/j.jinsphys.2003.09.011.

DOI:10.1016/j.jinsphys.2003.09.011

PMID:15037091

Abstract

Suppression-subtractive hybridization was used to isolate cDNAs specifically expressed in the brain at the termination of pupal diapause in Agrius convolvuli. One of the isolated clones shows similarity to the cytochrome c oxidase subunit 1 (COX1) gene. The full-length cDNA was obtained from brain mRNA by rapid amplification of cDNA ends (RACE). The insert is 1.65 kb in length and has an open reading frame of 1.46 kb which encodes a putative protein of 486 amino acid residues. RT-PCR reveals that the mRNA increases dramatically at an early stage of diapause termination. Activity of cytochrome c oxidase in the brain also increases at the same time. The up-regulation of this gene suggests that expression of the COX1 gene and ATP synthesis are initiated in the brain in association with diapause termination.

摘要

抑制消减杂交技术被用于分离在甘薯天蛾蛹滞育结束时大脑中特异性表达的cDNA。其中一个分离得到的克隆与细胞色素c氧化酶亚基1（COX1）基因具有相似性。通过cDNA末端快速扩增（RACE）从大脑mRNA中获得了全长cDNA。插入片段长度为1.65 kb，具有一个1.46 kb的开放阅读框，编码一个推定的由486个氨基酸残基组成的蛋白质。逆转录聚合酶链反应（RT-PCR）显示，在滞育结束的早期阶段，mRNA显著增加。大脑中细胞色素c氧化酶的活性也同时增加。该基因的上调表明，COX1基因的表达和ATP合成与滞育结束相关，在大脑中启动。