• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

Automated sequencing and mapping of cosmid DNA with fluorescently-labeled dideoxynucleotide terminators.

作者信息

Obermaier B, Domdey H

机构信息

Laboratorium für Molekulare Biologie, LMU München, FRG.

出版信息

Biotechniques. 1992 Jul;13(1):46-7.

PMID:1503773
Abstract

We have established a method for directly sequencing cosmid DNA on an automated DNA sequencer. The major advantage of this method is that only small amounts of cosmid template DNA are needed for the sequencing reactions.

摘要

相似文献

1
Automated sequencing and mapping of cosmid DNA with fluorescently-labeled dideoxynucleotide terminators.
Biotechniques. 1992 Jul;13(1):46-7.
2
Rapid restriction mapping of cosmids by sequence-specific triple-helix-mediated affinity capture.
Genomics. 1996 Jan 15;31(2):185-92. doi: 10.1006/geno.1996.0030.
3
Resolution of uncertainties in restriction maps of cosmid clones by "sequencing stitching".
Anal Biochem. 1995 Jul 1;228(2):355-7. doi: 10.1006/abio.1995.1365.
4
Dideoxy linear PCR on a commercial fluorescent automated DNA sequencer.在商用荧光自动DNA测序仪上进行双脱氧线性PCR。
Biotechniques. 1992 Jan;12(1):48-50, 52-3.
5
[A nested deletion method for cosmid DNA using the bacteriophage T3 in vitro packaging system].[一种利用噬菌体T3体外包装系统对黏粒DNA进行嵌套缺失的方法]
Tanpakushitsu Kakusan Koso. 1997 Jun;42(8):1327-31.
6
Non-isotopic restriction mapping of cosmid DNA.
Biotechniques. 1994 May;16(5):910-5.
7
Rapid restriction map constructions using a modified pWE15 cosmid vector and a robotic workstation.
Biotechniques. 1991 Aug;11(2):212-4, 216-7.
8
Sequence length and error analysis of Sequenase and automated Taq cycle sequencing methods.Sequenase和自动Taq循环测序方法的序列长度及误差分析
Biotechniques. 1993 Mar;14(3):442-7.
9
In vitro production of large single-stranded templates for DNA sequencing.用于DNA测序的大的单链模板的体外制备。
Biotechniques. 1991 Mar;10(3):376-84.
10
Genomic organization and DNA sequence of the human catecholamine-sulfating phenol sulfotransferase gene (STM).人类儿茶酚胺硫酸化酚硫酸转移酶基因(STM)的基因组结构与DNA序列
Biochem Biophys Res Commun. 1994 Dec 15;205(2):1325-32. doi: 10.1006/bbrc.1994.2810.