Gege Christian, Schneider Matthias F, Schumacher Gabriele, Limozin Laurent, Rothe Ulrich, Bendas Gerd, Tanaka Motomu, Schmidt Richard R
Universität Konstanz, Fach M 725 78457 Konstanz, Germany.
Chemphyschem. 2004 Feb 20;5(2):216-24. doi: 10.1002/cphc.200300947.
Functional microdomains of glycolipids were designed by mixing neoglycolipids with partially fluorinated alkyl (F-alkyl) chains and matrix lipids with alkyl chains. Fluorescence images of the mixed lipid monolayers at the air-water interface demonstrated that it is possible to control both size and distribution of the microdomains by means of the strong demixing of alkyl and F-alkyl membrane anchors, while the carbohydrate head groups seemed to play a rather minor role. These microdomains in monolayers could be transferred onto hydrophobized substrates and subjected to experiments in a dynamic flow chamber. The results obtained here clearly indicated that the dynamic adhesion of Chinese hamster ovarial cells expressing E-selectin (CHO-E cells) on a lipid monolayer containing microdomains of sialyl Lewisx (sLex) can be both enhanced and reduced by controlled demixing of ligands and matrices. Moreover, the same clusters of sLex could also be formed in giant lipid vesicles, which can be used as a model cell that locally expresses biospecific functions.
通过将新糖脂与部分氟化烷基（F-烷基）链混合以及将基质脂质与烷基链混合，设计了糖脂的功能微区。气-水界面混合脂质单层的荧光图像表明，通过烷基和F-烷基膜锚的强烈相分离，可以控制微区的大小和分布，而碳水化合物头部基团似乎起的作用较小。单层中的这些微区可以转移到疏水化的底物上，并在动态流动室中进行实验。此处获得的结果清楚地表明，通过配体和基质的可控相分离，可以增强或降低表达E-选择素的中国仓鼠卵巢细胞（CHO-E细胞）在含有唾液酸化路易斯x（sLex）微区的脂质单层上的动态粘附。此外，相同的sLex簇也可以在巨型脂质囊泡中形成，巨型脂质囊泡可作为局部表达生物特异性功能的模型细胞。
