Sauer L A, Dauchy R T
Cancer Research Laboratory, Mary Imogene Bassett Hospital, Cooperstown, New York 13326.
Br J Cancer. 1992 Aug;66(2):290-6. doi: 10.1038/bjc.1992.259.
The uptake of myristic (C14:0), palmitic (C16:0), palmitoleic (C:16,N-7), stearic (C18:0), oleic (C18:1,N-9), linoleic (C18:2,N-6) and arachidonic (C20:4,N-6) acids from plasma free fatty acids (FFA), triglycerides (TGA), phospholipids (PL) and cholesterol esters (CE) was measured in tissue-isolated hepatomas 7288CTC and 7777 in vivo. Adult tumour-bearing Buffalo rats were fed a normal chow diet ad libitum and were subjected to darkness from 1800 to 0600 h. Arterial plasma levels of FFA, TGA, PL and CE were increased during the dark period without change in fatty acid compositions. Arteriovenous difference measurements of tumour lipid uptake were performed between 0600 and 0900 h and included both high (dark) and low (light) arterial blood lipid concentrations. The rate of lipid uptake from each lipid class was directly dependent on the rate of supply of the lipid to the tumour. The efficiency of uptake, however, depended on the type of plasma lipid and the tumour. During one pass of arterial blood, hepatoma 7288CTC (n = 5 to 13) removed 46, 33, 36 and 31%, and hepatoma 7777 (n = 7 to 9) removed 48, 50, 52 and 49% of the fatty acids supplied in FFA, TGA, PL and CE, respectively. Perfusion of tissue-isolated tumours in situ with donor blood containing plasma free (1-14C)palmitic acid showed that 14C-palmitic acid was removed from the arterial blood and was incorporated into tumour lipids and that 14CO2 was released into the tumour venous blood. Uptake of the seven fatty acids over a 24 h period was greatest from PL greater than TGA greater than FFA greater than CE and was estimated to total 18.1 +/- 3.5 mg fatty acids g-1 for hepatoma 7288CTC and 25.9 +/- 3.5 mg fatty acids g-1 for hepatoma 7777. Both hepatoma 7288CTC and 7777 grew at a rate of about 1 g day-1 and contained 13.4 +/- 2.5 and 10.6 +/- 3.9 mg of these 7 fatty acids g-1 tumour wet weight, respectively. We conclude that these two tumours obtain all of the fatty acids needed for daily growth from host arterial blood.
在体内对组织分离的肝癌7288CTC和7777中肉豆蔻酸（C14:0）、棕榈酸（C16:0）、棕榈油酸（C16:1，n-7）、硬脂酸（C18:0）、油酸（C18:1，n-9）、亚油酸（C18:2，n-6）和花生四烯酸（C20:4，n-6）从血浆游离脂肪酸（FFA）、甘油三酯（TGA）、磷脂（PL）和胆固醇酯（CE）的摄取情况进行了测定。成年荷瘤布法罗大鼠随意喂食正常饲料，并在18:00至06:00处于黑暗环境。黑暗期动脉血浆中FFA、TGA、PL和CE水平升高，而脂肪酸组成无变化。在06:00至09:00期间进行肿瘤脂质摄取的动静脉差值测量，包括高（黑暗）和低（光照）动脉血脂浓度。每种脂质类别的脂质摄取率直接取决于脂质向肿瘤的供应速率。然而，摄取效率取决于血浆脂质类型和肿瘤。在动脉血单次通过期间，肝癌7288CTC（n = 5至13）分别摄取了FFA、TGA、PL和CE中供应的脂肪酸的46%、33%、36%和31%，肝癌7777（n = 7至9）分别摄取了48%、50%、52%和49%。用含游离（1-14C）棕榈酸血浆的供体血液原位灌注组织分离的肿瘤，结果显示14C-棕榈酸从动脉血中被摄取并掺入肿瘤脂质中，且14CO2释放到肿瘤静脉血中。在24小时内，七种脂肪酸的摄取量从PL大于TGA大于FFA大于CE，估计肝癌7288CTC为18.1±3.5毫克脂肪酸·克-1，肝癌7777为25.9±3.5毫克脂肪酸·克-1。肝癌7288CTC和7777均以约1克/天的速度生长，肿瘤湿重中每克分别含有13.4±2.5和10.6±3.9毫克这7种脂肪酸。我们得出结论，这两种肿瘤每日生长所需的所有脂肪酸均来自宿主动脉血。
