O'Neil Shawn P, Suwyn Carolyn, Anderson Daniel C, Niedziela Genevieve, Bradley Juliette, Novembre Francis J, Herndon James G, McClure Harold M
Division of Microbiology, Yerkes National Primate Research Center, Emory University School of Medicine, Atlanta, GA, USA.
Am J Pathol. 2004 Apr;164(4):1157-72. doi: 10.1016/S0002-9440(10)63204-X.
Infection of pig-tailed macaques with the simian immunodeficiency virus (SIV) isolate SIVsmmFGb frequently results in SIV encephalitis (SIVE) in addition to immunodeficiency and acquired immune deficiency syndrome. We used in situ hybridization to quantitate the number of SIV-infected cells in brain parenchyma, choroid plexus, and meninges from 17 macaques that developed acquired immune deficiency syndrome after infection with SIVsmmFGb. SIV-infected cells and histopathological lesions of SIVE were identified in 15 of 17 animals (88.2%), including 12 of 12 rapid progressors (RP) and 3 of 5 slow progressors (SP). The parenchymal virus burden was much greater in RP macaques than in the three SP macaques with SIVE (median values of 24.3 versus 0.3 infected cells/mm(2), respectively; P < 0.05). Viral load differences between RP and SP with SIVE were less marked in choroid plexus (29.6 versus 12.8 infected cells/mm(2), respectively) and meninges (133.0 versus 34.2 infected cells/mm(2), respectively). A significant negative correlation was observed between the magnitude of the anti-SIV antibody titer at 1 month after inoculation and brain virus burden at necropsy (r = -0.614; P < 0.01). The close association between immune response and SIVE in this model should prove useful for identifying correlates of immune protection against primate lentiviral encephalitis.
将猪尾猕猴感染猿猴免疫缺陷病毒（SIV）毒株SIVsmmFGb后，除了会出现免疫缺陷和获得性免疫缺陷综合征外，还经常导致SIV脑炎（SIVE）。我们使用原位杂交技术对17只感染SIVsmmFGb后出现获得性免疫缺陷综合征的猕猴的脑实质、脉络丛和脑膜中SIV感染细胞的数量进行定量分析。在17只动物中的15只（88.2%）中鉴定出SIV感染细胞和SIVE的组织病理学病变，其中包括12只快速进展者（RP）中的12只和5只缓慢进展者（SP）中的3只。RP猕猴的实质病毒载量比三只患有SIVE的SP猕猴高得多（中位数分别为24.3个感染细胞/mm²和0.3个感染细胞/mm²；P<0.05）。在脉络丛（分别为29.6个感染细胞/mm²和12.8个感染细胞/mm²）和脑膜（分别为133.0个感染细胞/mm²和34.2个感染细胞/mm²）中，患有SIVE的RP和SP之间的病毒载量差异不太明显。在接种后1个月时抗SIV抗体滴度的大小与尸检时脑病毒载量之间观察到显著的负相关（r = -0.614；P<0.01）。在该模型中免疫反应与SIVE之间的密切关联应有助于确定针对灵长类慢病毒脑炎的免疫保护相关因素。
