He Ya-ping, Li Zhuo-ya, Jiang Xiao-dan, Feng Wei, Xu Yong, Xiong Ping
Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Yao Xue Xue Bao. 2003 Dec;38(12):889-92.
To study the effects of TNF receptor blocking peptide on adjuvant arthritis in rats.
The model of rat adjuvant arthritis was induced by injection of complete Freund's adjuvant. The TNF receptor blocking peptide was injected locally in the ankle. The ankle swelling, the pathologic changes in the ankle joint and the expression of IL-1 beta mRNA and TNF-alpha mRNA by peritoneal macrophages (RT-PCR) were observed.
The model of rat adjuvant arthritis induced by injection of complete Freund's adjuvant was similar to human rheumatoid arthritis. The treatment with TNF receptor blocking peptide for 10 days resulted in complete inhibition of joint swelling, a decrease in infiltration of inflammatory cell into joint tissue, an obvious alleviation of inflammatory pathological damages and an apparent decline of TNF-alpha mRNA and IL-1 beta mRNA of peritoneal macrophages of rats.
The TNF receptor blocking peptide can protect the joint from inflammatory damage induced by adjuvant arthritis by suppression of TNF-alpha and IL-1 production, thereby alleviating the pathological injury of joint and controlling effectively the clinic course of arthritis.
研究肿瘤坏死因子（TNF）受体阻断肽对大鼠佐剂性关节炎的影响。
通过注射完全弗氏佐剂诱导大鼠佐剂性关节炎模型。将TNF受体阻断肽局部注射到踝关节。观察踝关节肿胀、踝关节的病理变化以及腹膜巨噬细胞中白细胞介素-1β（IL-1β）mRNA和肿瘤坏死因子-α（TNF-α）mRNA的表达（逆转录-聚合酶链反应法）。
注射完全弗氏佐剂诱导的大鼠佐剂性关节炎模型与人类类风湿关节炎相似。用TNF受体阻断肽治疗10天可导致关节肿胀完全受到抑制，炎性细胞向关节组织的浸润减少，炎性病理损伤明显减轻，大鼠腹膜巨噬细胞的TNF-α mRNA和IL-1β mRNA明显下降。
TNF受体阻断肽可通过抑制TNF-α和IL-1的产生保护关节免受佐剂性关节炎诱导的炎性损伤，从而减轻关节的病理损伤并有效控制关节炎的临床病程。
