单价阳离子对酵母去极化作用的浓度依赖性。
The concentration dependence of the depolarization of yeast by monovalent cations.
作者信息
Borst-Pauwels G W, van de Mortel J B, Theuvenet A P
机构信息
Laboratory of Cell Biology, Catholic University, Faculty of Science, Nijmegen, Netherlands.
出版信息
Biochim Biophys Acta. 1992 Jun 30;1107(2):207-12. doi: 10.1016/0005-2736(92)90406-c.
Monovalent cations decrease the initial rate of uptake of the membrane potential probe 2-(dimethylaminostyryl)-1-ethyl-pyridinium (DMP) into metabolizing cells, showing that the cells are depolarized. A steep decrease in this rate was found even at low cation concentrations, reaching 62%, 42%, 58%, 40% and 40% at high concentrations of K+, Rb+, Cs+, Na+ and Li+, respectively. The corresponding concentrations at which half-maximum decrease was found were 0.22, 0.36, 1.2, 17 and 17 mM. These values are of the same order of magnitude as the half-saturation concentrations for monovalent cation uptake by the yeast.
单价阳离子会降低膜电位探针2-（二甲基氨基苯乙烯基）-1-乙基吡啶鎓（DMP）进入代谢细胞的初始摄取速率，这表明细胞发生了去极化。即使在低阳离子浓度下，也发现该速率急剧下降，在高浓度的K⁺、Rb⁺、Cs⁺、Na⁺和Li⁺下分别达到62%、42%、58%、40%和40%。发现半数最大下降时的相应浓度分别为0.22、0.36、1.2、17和17 mM。这些值与酵母摄取单价阳离子的半饱和浓度处于同一数量级。
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