Matejusová I, Cunningham C O
FRS Marine Laboratory, P.O. Box 101, Victoria Road, Aberdeen AB11 9DB, UK.
J Parasitol. 2004 Feb;90(1):146-51. doi: 10.1645/GE-111R3.
The sequence of the Gyrodactylus salaris Malmberg, 1957, large subunit, or 28S, ribosomal RNA (rRNA) gene has been determined. This gene is the final portion of the Gyrodactylus rRNA gene operon to be sequenced and results in the first complete sequence of all rRNA genes and spacers from a monogenean. The nucleotide sequence was used to predict the secondary structure of the large subunit rRNA, and regions of conserved and variable sequence and structure were identified. The site where the 5' terminus of the 5.8S rRNA binds to a region within the large subunit rRNA was predicted and complements the anticipated interaction of the 3' terminus of the 5.8S with the 5' terminus of the large subunit rRNA. The large subunit gene may be useful in phylogenetic analysis of the Monogenea or Platyhelminthes and comparisons with other eukaryotes. The variable domains C and H may be most suitable for this purpose.
已确定1957年马尔姆贝格的鲑三代虫（Gyrodactylus salaris）的大核糖体亚基（即28S）核糖体RNA（rRNA）基因的序列。该基因是三代虫rRNA基因操纵子中最后一个被测序的部分，从而得到了单殖吸虫所有rRNA基因及间隔区的首个完整序列。利用该核苷酸序列预测了大核糖体亚基rRNA的二级结构，并识别出保守及可变的序列和结构区域。预测了5.8S rRNA的5′末端与大核糖体亚基rRNA内一个区域结合的位点，该位点与预期的5.8S的3′末端和大核糖体亚基rRNA的5′末端之间的相互作用互补。大核糖体亚基基因可能有助于单殖吸虫或扁形动物门的系统发育分析，以及与其他真核生物的比较。可变结构域C和H可能最适合用于此目的。
