Buzin Yuri, Carrasco Nicolas, Huang Zhen
Department of Chemistry, Brooklyn College, The Graduate School, The City University of New York, 2900 Bedford Avenue, Brooklyn, New York 11210, USA.
Org Lett. 2004 Apr 1;6(7):1099-102. doi: 10.1021/ol0365077.
Synthesis of the novel 2'-Se-cytidine phosphoramidite was achieved via transformation of the uridine analogue to the cytidine derivative in high yield. This 2'-Se-cytidine phosphoramidite was used to synthesize selenium-derivatized DNA and RNA oligonucleotides for X-ray crystallography using MAD. The nucleotide coupling yield using this novel phosphoramidite was over 99% when 5-benzylmercaptotetrazole (5-BMT) was used as the coupling reagent.
通过将尿苷类似物高产率转化为胞苷衍生物，实现了新型2'-硒代胞苷亚磷酰胺的合成。该2'-硒代胞苷亚磷酰胺用于使用多波长反常散射（MAD）的X射线晶体学，合成硒衍生化的DNA和RNA寡核苷酸。当使用5-苄基巯基四唑（5-BMT）作为偶联试剂时，使用这种新型亚磷酰胺的核苷酸偶联产率超过99%。
