Renker Carsten, Blanke Verena, Börstler Boris, Heinrichs Jochen, Buscot François
Terrestrial Ecology, Institute of Botany, University of Leipzig, Johannisallee 21, D-04103 Leipzig, Germany.
FEMS Yeast Res. 2004 Mar;4(6):597-603. doi: 10.1016/j.femsyr.2004.01.001.
The genera Cryptococcus and Dioszegia contain basidiomycetous yeasts found in a wide range of habitats. Primers to amplify the internal transcribed spacer (ITS) region of the nuclear ribosomal DNA (nrDNA) of arbuscular mycorrhizal fungi (AMF) also allow detecting members of this yeast group. Here we report the results of a sequence analysis using maximum parsimony on a set of 50 ITS sequences of yeasts associated with AMF structures (roots of 26 plant species, AM spores) from six field sites in Central Germany. Among 10 separated taxa, respectively five in the Tremellales and two in the Filobasidiales had unknown sequences. Therefore it was not possible to assign these sequences to any known species. The study indicates that exploring the diversity of Cryptococcus and Dioszegia in soil habitats with molecular methods might enlarge the actually estimated biodiversity of the group.
隐球菌属和迪氏酵母属包含在广泛生境中发现的担子菌酵母。用于扩增丛枝菌根真菌（AMF）核糖体DNA（nrDNA）内部转录间隔区（ITS）的引物也可用于检测该酵母类群的成员。在此，我们报告了使用最大简约法对来自德国中部六个野外地点与AMF结构（26种植物的根、AM孢子）相关的50个酵母ITS序列进行序列分析的结果。在10个分离的分类单元中，银耳目分别有5个，线黑粉菌目有2个具有未知序列。因此，无法将这些序列归为任何已知物种。该研究表明，用分子方法探索土壤生境中隐球菌属和迪氏酵母属的多样性可能会扩大该类群实际估计的生物多样性。
