Khwantongyim Punda, Wansee Somying, Lu Xi, Zhang Wei, Sun Guangyu
State Key Laboratory of Crop Stress Biology in Arid Areas, College of Plant Protection, Northwest A&F University, Yangling 712100, China.
J Fungi (Basel). 2021 Sep 15;7(9):764. doi: 10.3390/jof7090764.
The various fungal communities that adhere to apple fruit are influenced by agricultural practices. However, the effects of fruit bagging-based management practice on the fungal microbiota are still unknown, and little is known about the fungal communities of bagged apple fruit. We conducted a study using apple fruit grown in a conventionally managed orchard where pesticide use is an indispensable practice. Fungal communities were collected from the calyx-end and peel tissues of bagged and unbagged fruit and characterized using barcode-type next-generation sequencing. Fruit bagging had a stronger effect on fungal richness, abundance, and diversity of the fungal microbiota in comparison to non-bagging. In addition, bagging also impacted the compositional variation of the fungal communities inhabiting each fruit part. We observed that fruit bagging had a tendency to maintain ecological equilibrium since Ascomycota and Basidiomycota were more distributed in bagged fruit than in unbagged fruit. These fungal communities consist of beneficial fungi rather than potentially harmful fungi. Approximately 50 dominant taxa were detected in bagged fruit, for example, beneficial genera such as , , , , and , as well as pathogenic genera such as and . These results suggested that fruit bagging could significantly increase fungal richness and promote healthy fungal communities, especially the harmless fungal communities, which might be helpful for protecting fruit from the effects of pathogens. This study provides a foundation for understanding the impacts of bagging-based practice on the associated fungal microbiota.
附着在苹果果实上的各种真菌群落会受到农业实践的影响。然而，基于果实套袋的管理措施对真菌微生物群的影响仍不明确，对于套袋苹果果实的真菌群落也知之甚少。我们对在传统管理果园中种植的苹果果实进行了一项研究，在该果园中，农药使用是一项必不可少的措施。从套袋和未套袋果实的萼端和果皮组织中采集真菌群落，并使用条形码型下一代测序对其进行表征。与不套袋相比，果实套袋对真菌微生物群的真菌丰富度、丰度和多样性有更强的影响。此外，套袋还影响了栖息在每个果实部位的真菌群落的组成变化。我们观察到，果实套袋有维持生态平衡的趋势，因为子囊菌门和担子菌门在套袋果实中的分布比未套袋果实中更多。这些真菌群落由有益真菌而非潜在有害真菌组成。在套袋果实中检测到约50个优势分类单元，例如有益属如 、 、 、 、 和 ，以及致病属如 和 。这些结果表明，果实套袋可显著增加真菌丰富度并促进健康的真菌群落，尤其是无害真菌群落，这可能有助于保护果实免受病原体的影响。本研究为理解基于套袋的措施对相关真菌微生物群的影响提供了基础。
