Parella Teodor
Servei de Ressonància Magnètica Nuclear, Universitat Autònoma de Barcelona, E-08193, Bellaterra, Barcelona, Spain.
J Magn Reson. 2004 Apr;167(2):266-72. doi: 10.1016/j.jmr.2004.01.002.
Several heteronuclear coherence transfer mechanisms involved in proton-detected heteronuclear J-cross-polarization (HCP) NMR experiments have been theoretically derived and experimentally verified in isotropic solution. It is shown that in-phase and/or anti-phase heteronuclear coherence transfer can take place separately or simultaneously during the HCP process as a function of the relative phase between the HCP mixing sequence and the corresponding input magnetization. As the more important consequence, clean coherence-order and spin-state selective (S3) excitation with maximum sensitivity can be achieved from gradient-enhanced HCP experiments by proper co-addition/subtraction of in-phase and anti-phase magnetizations, offering an attractive alternative to widely used HSQC-type experiments.
质子检测的异核J交叉极化（HCP）核磁共振实验中涉及的几种异核相干转移机制已在理论上推导出来，并在各向同性溶液中得到实验验证。结果表明，在HCP过程中，同相和/或反相异核相干转移可以根据HCP混合序列与相应输入磁化强度之间的相对相位分别或同时发生。更重要的是，通过对同相和反相磁化强度进行适当的相加/相减，梯度增强的HCP实验可以实现具有最大灵敏度的纯净相干序和自旋态选择性（S3）激发，为广泛使用的HSQC型实验提供了一种有吸引力的替代方案。
