Pelupessy P, Chiarparin E, Bodenhausen G
Section de Chimie, Universite de Lausanne, Lausanne, 1015, Switzerland.
J Magn Reson. 1999 May;138(1):178-81. doi: 10.1006/jmre.1999.1715.
In isotopically labeled macromolecules, it is possible to excite the signal of a selected proton by shuttling magnetization back and forth between the chosen proton and a heteronucleus such as 13C or 15N, using two-way doubly selective heteronuclear cross-polarization. Selective excitation of a chosen proton can be followed by homonuclear coherence transfer to identify side-chain resonances of the corresponding amino acid in proteins. The resulting one-dimensional experiments yield information that can usually only be obtained from three-dimensional HSQC-TOCSY spectra. The method also provides efficient suppression of solvent signals without affecting resonances close to the solvent peak. Copyright 1999 Academic Press.
在同位素标记的大分子中，利用双向双选择性异核交叉极化，通过在选定的质子与诸如(^{13}C)或(^{15}N)等异核之间来回传递磁化强度，有可能激发选定质子的信号。选定质子的选择性激发之后可以进行同核相干转移，以识别蛋白质中相应氨基酸的侧链共振。由此产生的一维实验所提供的信息通常只能从三维HSQC - TOCSY谱中获得。该方法还能有效抑制溶剂信号，而不影响靠近溶剂峰的共振信号。版权所有1999年，学术出版社。
