Reing Janet E, Gollin Susanne M, Saunders William S
Department of Biological Sciences, University of Pittsburgh and the University of Pittsburgh Cancer Institute, Pittsburgh, PA 15260, USA.
Cancer Genet Cytogenet. 2004 Apr 1;150(1):57-61. doi: 10.1016/j.cancergencyto.2003.08.002.
Chromosomal segregational defects are commonly observed in cancer cells and are an important source of genetic instability. It is currently unknown whether these mitotic defects are the result of a subpopulation of defective cells or reflect characteristics of the population of cells as a whole. In this study, we compared chromosomal segregational defects in two oral squamous cell carcinoma cell lines and five single-cell clones from each of those cell lines. We used immunofluorescence microscopy to quantitate the occurrence of multipolar metaphase spindles, lagging chromosomes at metaphase and anaphase, and anaphase bridges. We conclude that chromosome segregational defects in these cancer cell lines represent an intrinsic and inherited tendency toward segregational defects in the general cell population, rather than the existence of a subpopulation of cells with segregational defects.
染色体分离缺陷在癌细胞中普遍存在，是遗传不稳定性的重要来源。目前尚不清楚这些有丝分裂缺陷是部分缺陷细胞的结果，还是反映了整个细胞群体的特征。在本研究中，我们比较了两种口腔鳞状细胞癌细胞系以及来自这些细胞系的五个单细胞克隆中的染色体分离缺陷。我们使用免疫荧光显微镜来定量多极中期纺锤体、中期和后期滞后染色体以及后期桥的出现情况。我们得出结论，这些癌细胞系中的染色体分离缺陷代表了一般细胞群体中存在的内在和遗传的分离缺陷倾向，而不是存在具有分离缺陷的细胞亚群。
