Li J, Helmerhorst E J, Yao Y, Nunn M E, Troxler R F, Oppenheim F G
Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston University, 700 Albany Street, Suite W201, Boston, MA 02118, USA.
Arch Oral Biol. 2004 May;49(5):379-85. doi: 10.1016/j.archoralbio.2004.01.002.
Recently, we demonstrated that anti-statherin monoclonal antibodies could be generated upon immunisation of mice with in vivo formed human acquired enamel pellicle, indicating that statherin is a constituent of pellicle. To gain insight in the in vivo adsorption behaviour of statherin we tested the abundance of statherin in pellicle and investigated the relationship between statherin and protein levels in salivary secretions and pellicle using a capture ELISA. Statherin levels were approximately 20-fold higher in parotid and submandibular-sublingual secretions than in cleared whole saliva supernatant or pellicle, suggesting the rapid degradation of statherin in the oral cavity. A strong positive correlation was observed between statherin and protein levels in pellicle but not in saliva indicating that statherin and protein adsorption to pellicle are related processes. This indicates that statherin represents the integral part of proteins that constitute the pellicle structure and may play a key role in its formation.
最近，我们证明在用体内形成的人获得性釉质 pellicle 免疫小鼠后可产生抗 statherin 单克隆抗体，这表明 statherin 是 pellicle 的一种成分。为深入了解 statherin 在体内的吸附行为，我们检测了 pellicle 中 statherin 的丰度，并使用捕获 ELISA 研究了 statherin 与唾液分泌物及 pellicle 中蛋白质水平之间的关系。腮腺和颌下 - 舌下分泌物中的 statherin 水平比清除后的全唾液上清液或 pellicle 中的高约 20 倍，这表明 statherin 在口腔中迅速降解。在 pellicle 中观察到 statherin 与蛋白质水平之间存在强正相关，但在唾液中未观察到，这表明 statherin 和蛋白质吸附到 pellicle 是相关过程。这表明 statherin 是构成 pellicle 结构的蛋白质的组成部分，可能在其形成中起关键作用。
