Jensen J L, Lamkin M S, Oppenheim F G
Department of Periodontology and Oral Biology, Boston University School of Graduate Dentistry, Massachusetts 02118.
J Dent Res. 1992 Sep;71(9):1569-76. doi: 10.1177/00220345920710090501.
The protein compositions of in vitro pellicles formed from whole saliva and parotid and submandibular secretions were determined by use of synthetic hydroxyapatite as a model for dental enamel. The adsorbed and unadsorbed protein fractions were analyzed by amino acid analysis and both anionic and cationic discontinuous polyacrylamide gel electrophoresis. For further characterization of the in vitro pellicle, the adsorbed fractions were subjected to gel filtration on Sephadex G-100 and reversed-phase chromatography on C18 columns. Amylase, acidic and glycosylated proline-rich proteins, statherins, and histatins were identified in the parotid-derived pellicle. Detailed analysis of the statherin-containing fractions resulted in the observation of several statherin-like proteins. The use of cationic gel electrophoresis allowed for the identification of histatin 3 and histatin 5, which have not been previously detected in pellicle formed in vitro. The protein composition of submandibular-derived pellicle was similar to that of parotid-derived pellicle except for the presence of cystatins and the absence of glycosylated proline-rich proteins. In contrast, in vitro pellicle derived from whole saliva exhibited a vastly different composition, consisting primarily of amylase, acidic proline-rich proteins, cystatins, and proteolytically-derived peptides. The results indicate that acidic phosphoproteins as well as neutral and basic histatins from pure secretions selectively adsorb to hydroxyapatite, whereas in whole saliva some of these proteins are proteolytically degraded, dramatically changing its adsorption pattern.
以合成羟基磷灰石作为牙釉质模型，测定了由全唾液、腮腺分泌液和颌下腺分泌液形成的体外菌斑的蛋白质组成。通过氨基酸分析以及阴离子和阳离子不连续聚丙烯酰胺凝胶电泳对吸附和未吸附的蛋白质组分进行了分析。为了进一步表征体外菌斑，对吸附组分进行了Sephadex G - 100凝胶过滤和C18柱反相色谱分析。在腮腺来源的菌斑中鉴定出了淀粉酶、酸性和糖基化富含脯氨酸的蛋白质、富组蛋白和组胺。对含富组蛋白的组分进行详细分析后，观察到了几种类富组蛋白。阳离子凝胶电泳的使用使得能够鉴定出富组蛋白3和富组蛋白5，这两种蛋白此前在体外形成的菌斑中未被检测到。颌下腺来源的菌斑的蛋白质组成与腮腺来源的菌斑相似，只是存在胱抑素且不存在糖基化富含脯氨酸的蛋白质。相比之下，全唾液来源的体外菌斑表现出截然不同的组成，主要由淀粉酶、酸性富含脯氨酸的蛋白质、胱抑素和蛋白水解衍生的肽组成。结果表明，纯分泌液中的酸性磷蛋白以及中性和碱性富组蛋白会选择性吸附到羟基磷灰石上，而在全唾液中，其中一些蛋白质会被蛋白水解降解，从而显著改变其吸附模式。
