Kawasaki Takashi, Kawasaki Chika, Ogata Masanori, Shigematsu Akio
Department of Anesthesiology, University of Occupational and Environmental Health, Kitakyushu, Japan.
Anesth Analg. 2004 Apr;98(4):1024-1029. doi: 10.1213/01.ANE.0000104480.04856.93.
It has been demonstrated that local anesthetics have several effects on the immune system. Monocytes and macrophages are essential components of the host response to microbial infection; however, the effect of local anesthetics on monocyte surface receptor expression remains unclear. We designed this study to investigate the effects of local anesthetics on monocyte mCD14 and human leukocyte antigen (HLA)-DR expression and lipopolysaccharide (LPS)-induced or staphylococcal enterotoxin B (SEB)-induced tumor necrosis factor (TNF)-alpha production. Blood samples were obtained from 10 healthy volunteers. The effects of local anesthetics on LPS- or SEB-induced TNF-alpha production were determined by using an enzyme-linked immunosorbent assay. After different doses of local anesthetics were added, the blood was stimulated with LPS (10 ng/mL) or SEB (10 micro g/mL) for 4 h. The effects of local anesthetics on monocyte mCD14 and HLA-DR expression were measured by dual monoclonal antibody staining and flow cytometry. Local anesthetics showed no effect on LPS- or SEB-induced TNF-alpha production in human whole blood. Local anesthetics suppressed monocyte HLA-DR expression in a dose-dependent manner (P < 0.05) but had no effect on monocyte mCD14 expression. This study demonstrated that local anesthetics suppress HLA-DR expression on the surface of human monocytes.
Monocyte surface receptors have a crucial role in the host response to microbial infection. We investigated the effects of local anesthetics on monocyte mCD14 and human leukocyte antigen (HLA)-DR expression. Our results show that local anesthetics suppress HLA-DR expression on the surface of human monocytes.
已证明局部麻醉药对免疫系统有多种作用。单核细胞和巨噬细胞是宿主对微生物感染反应的重要组成部分；然而，局部麻醉药对单核细胞表面受体表达的影响仍不清楚。我们设计本研究以调查局部麻醉药对单核细胞mCD14和人类白细胞抗原（HLA）-DR表达以及脂多糖（LPS）诱导或葡萄球菌肠毒素B（SEB）诱导的肿瘤坏死因子（TNF）-α产生的影响。从10名健康志愿者采集血样。使用酶联免疫吸附测定法确定局部麻醉药对LPS或SEB诱导的TNF-α产生的影响。加入不同剂量的局部麻醉药后，用LPS（10 ng/mL）或SEB（10 μg/mL）刺激血液4小时。通过双单克隆抗体染色和流式细胞术测量局部麻醉药对单核细胞mCD14和HLA-DR表达的影响。局部麻醉药对人全血中LPS或SEB诱导的TNF-α产生无影响。局部麻醉药以剂量依赖性方式抑制单核细胞HLA-DR表达（P < 0.05），但对单核细胞mCD14表达无影响。本研究表明局部麻醉药抑制人单核细胞表面的HLA-DR表达。
单核细胞表面受体在宿主对微生物感染的反应中起关键作用。我们研究了局部麻醉药对单核细胞mCD14和人类白细胞抗原（HLA）-DR表达的影响。我们的结果表明局部麻醉药抑制人单核细胞表面的HLA-DR表达。
