Zelenin A V
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.
Mol Biol (Mosk). 2004 Jan-Feb;38(1):17-28.
Physical chromosome mapping by fluorescence in situ hybridization (FISH) is among the major lines of research on the human genome (as well as genomes of numerous other organisms). To localize particular genes or anonymous DNA sequences on individual chromosomes or chromosome regions, FISH was developed in the late 1980s and early 1990s, when the International Human Genome Project and the Russian program Human Genome were launched. Now FISH continues to play a prominent part in studies of the human genome. The review considers the major steps of FISH development in Russia with special emphasis on the key roles of the Institute of Cytology and Genetics (Novosibirsk) and Engelhardt Institute of Molecular Biology (Moscow). Physical mapping of human chromosomes 3 and 13 by FISH is described in detail. The promotion of FISH in Russia contributed to the progress in the related fields such as comparative animal genomics (ZOO-FISH) and studies of plant chromosomes.
通过荧光原位杂交（FISH）进行物理染色体图谱分析是人类基因组（以及许多其他生物的基因组）主要研究方向之一。为了将特定基因或无名DNA序列定位到单个染色体或染色体区域，FISH技术于20世纪80年代末90年代初得以开发，当时国际人类基因组计划和俄罗斯人类基因组计划启动。如今，FISH技术在人类基因组研究中仍发挥着重要作用。本综述探讨了FISH技术在俄罗斯发展的主要阶段，特别强调了新西伯利亚细胞学与遗传学研究所和莫斯科恩格尔哈特分子生物学研究所所发挥的关键作用。详细介绍了通过FISH对人类3号和13号染色体进行物理图谱分析的情况。FISH技术在俄罗斯的推广推动了比较动物基因组学（动物荧光原位杂交）和植物染色体研究等相关领域的发展。
