Heng H H, Spyropoulos B, Moens P B
Department of Biology, York University, Downsview, Ontario, Canada.
Bioessays. 1997 Jan;19(1):75-84. doi: 10.1002/bies.950190112.
Fluorescent in situ hybridization technology is one of the most exciting and versatile research tools to be developed in recent years. It has enabled research to progress at a phenomenal rate in diverse areas of basic research as well as in clinical medicine. Fluorescent in situ hybridization has applications in physical mapping, the study of nuclear architecture and chromatin packaging, and the investigation of fundamental principles of biology such as DNA replication, RNA processing, gene amplification, gene integration and chromatin elimination. This review highlights some of these areas and provides source material for the reader who seeks more information on a specific field.
荧光原位杂交技术是近年来开发的最令人兴奋且用途广泛的研究工具之一。它使基础研究和临床医学等不同领域的研究得以以惊人的速度推进。荧光原位杂交技术在物理图谱绘制、核结构和染色质包装研究，以及DNA复制、RNA加工、基因扩增、基因整合和染色质消除等生物学基本原理的研究中均有应用。本综述重点介绍了其中一些领域，并为寻求特定领域更多信息的读者提供了参考资料。
