Hubel Allison, Carlquist Dale, Clay Mary, McCullough Jeff
Department of Mechanical Engineering, University of Minnesota, Minneapolis 55455, USA.
Transfusion. 2004 Apr;44(4):518-25. doi: 10.1111/j.1537-2995.2004.03238.x.
Cord blood banking requires methods for shipping and storage. This study examines the influence of shipping via overnight courier on postthaw viability of cord blood.
Anticoagulated cord blood was divided with one sample diluted 1:1 using STM-sav (a storage solution) and the other undiluted. Units were shipped from Minneapolis to Memphis and returned, RBC-depleted, cryopreserved, stored for 14 days, and thawed. MNC counts, percent viable cells, quantity of CD34+ cells, and frequency of CFU-GM were measured. Temperature during shipment was continuously monitored.
Preliminary studies showed the packing and processing protocol influenced the temperatures experienced during shipping. Samples achieved temperatures below 10 degrees C within 4 to 8 hours with a few units dropping near or below 1 degrees C with cold ambient temperatures. The MNC recovery, CD34+45+ recovery, and frequency of CFU-GM for samples that were shipped were comparable to those observed using static liquid storage. The postthaw viable cell recovery was greatest for storage and shipping times of 24 hours and decreased when the storage and shipping times were longer.
Ambient conditions and the packing and processing protocol influence the temperature history of the sample. Samples stored beyond 24 hours in liquid storage and shipping exhibit a decreased postthaw recovery.
脐血库需要运输和储存方法。本研究探讨通过隔夜快递运输对脐血解冻后活力的影响。
将抗凝脐血进行分割，一份样本用STM-sav（一种储存溶液）按1:1稀释，另一份不稀释。样本从明尼阿波利斯运往孟菲斯，然后返回，去除红细胞后冷冻保存14天，再进行解冻。测量单核细胞计数、活细胞百分比、CD34+细胞数量和粒系巨噬系集落形成单位（CFU-GM）频率。运输过程中的温度进行持续监测。
初步研究表明包装和处理方案会影响运输过程中经历的温度。样本在4至8小时内温度降至10摄氏度以下，少数样本在寒冷环境温度下接近或低于1摄氏度。运输样本的单核细胞回收率、CD34+45+回收率和CFU-GM频率与静态液体储存观察到的结果相当。解冻后活细胞回收率在储存和运输时间为24小时时最高，当储存和运输时间更长时则下降。
环境条件以及包装和处理方案会影响样本的温度历程。在液体储存和运输中超过24小时储存的样本解冻后回收率降低。
