Saxena Vishal, Sadoqi Mostafa, Shao Jun
Department of Pharmacy and Administrative Sciences, College of Pharmacy and Allied Health Professions, St. John's University, 8000 Utopia Parkway, Jamaica, NY 11439, USA.
J Photochem Photobiol B. 2004 Mar 19;74(1):29-38. doi: 10.1016/j.jphotobiol.2004.01.002.
Photo-degradation, thermal-degradation and aqueous-instability of indocyanine green (ICG) limits its application as a fluorescence contrast agent for imaging purposes. Thus, the objective of this study is to develop polymeric nanoparticles entrapping ICG and to establish its effectiveness in providing photo-stability, thermal stability and aqueous stability to ICG. Nanoparticles entrapping ICG were engineered, characterized and the degradation kinetics of ICG in the nanoparticles was investigated in aqueous media. The entrapment of ICG in the nanoparticles causes a shift in its wavelength of peak fluorescence and a decrease in its peak fluorescence intensity. The degradation of ICG in aqueous nanoparticle suspension followed first-order kinetics for the time period studied. ICG entrapment in the nanoparticles enhanced aqueous-stability of ICG (half-life, t(1/2) was 72.2+/-6.1 h for ICG in the nanoparticles as compared to 16.8+/-1.5 h for free ICG solution), photo-stability of ICG (t(1/2) was 73.7+/-7.5 h for ICG in the nanoparticles as compared to 14.4+/-2.4 h for free ICG solution when exposed to room light from two 32 W normal fluorescent tubes) and thermal-stability of ICG (t(1/2) of ICG at 42 degrees C was 62.4+/-1.7 h for ICG in the nanoparticles as compared to 10.1+/-0.6 h for free ICG solution).
吲哚菁绿（ICG）的光降解、热降解和水相不稳定性限制了其作为荧光造影剂用于成像目的。因此，本研究的目的是开发包载ICG的聚合物纳米颗粒，并确定其在为ICG提供光稳定性、热稳定性和水相稳定性方面的有效性。对包载ICG的纳米颗粒进行了设计、表征，并研究了ICG在纳米颗粒中的降解动力学。ICG包载于纳米颗粒中会导致其荧光峰值波长发生偏移，并使其峰值荧光强度降低。在研究时间段内，ICG在纳米颗粒水悬浮液中的降解遵循一级动力学。纳米颗粒中包载ICG增强了ICG的水相稳定性（纳米颗粒中ICG的半衰期t(1/2)为72.2±6.1小时，而游离ICG溶液的半衰期为16.8±1.5小时）、光稳定性（当暴露于两根32W普通荧光灯管的室内光线下时，纳米颗粒中ICG的t(1/2)为73.7±7.5小时，而游离ICG溶液的t(1/2)为14.4±2.4小时）以及热稳定性（42℃时纳米颗粒中ICG的t(1/2)为62.4±1.7小时，而游离ICG溶液的t(1/2)为10.1±0.6小时）。
