Sakamoto Ken-Ichi, Mori Yoshio, Takagi Hisato, Iwata Hisashi, Yamada Takuya, Futamura Naoki, Sago Tetsu, Ezaki Takayuki, Kawamura Yoshiaki, Hirose Hajime
First Department of Surgery, Gifu University School of Medicine, Gifu, Japan.
Nutrition. 2004 Apr;20(4):372-6. doi: 10.1016/j.nut.2003.12.010.
We tested whether alterations in intestinal morphology and mucus gel correlate with differences in Salmonella typhimurium translocation between rats treated with total parenteral nutrition (TPN) and rats given a diet of chow.
Twenty-seven male Wistar rats were assigned to one of two groups: one received TPN for 14 d and the other (control) received standard rat chow and water ad libitum. Salmonella typhimurium (5 x 10(8) cells; GIFU 12142) was injected into a closed ileal loop. Portal venous blood (PVB), inferior vena cava blood (IVCB), and mesenteric lymph nodes (MLNs) were sampled for evaluation of bacterial translocation. Sections of the loop were prepared and stained with hematoxylin and eosin, periodic acid-Schiff (PAS), and fluorescein isothiocyanate-labeled Ulex europaeus agglutinin I (FITC-UEA-I) for image analysis. Perimeter, mucosal thickness, villus area, and positively stained mucus area were measured. A fluorescent antibody study was also done.
Organisms were found in cultures of 1 in 13 control rats and 9 in 14 TPN rats. There were more bacteria in MLNs than in PVB or IVCB. There was no increase in the number of bacteria over time in PVB, IVCB, or MLNs. Perimeter and villus area (P < 0.001) and mucosal thickness (P < 0.01) were significantly smaller in the TPN group than in the control group. The positively stained mucus area was significantly smaller in the TPN group than in the control group (P < 0.05 with PAS, P < 0.01 with FITC-UEA-I). Salmonella typhimurium invaded specifically through Peyer's patches. In all culture-negative samples, bacteria were trapped by the mucous layer, with a very small number attached to the epithelial surface.
Significant villous atrophy and reduction of mucus play an important role in the rapid translocation of S. typhimurium through Peyer's patches in rats after 2 wk of TPN.
我们检测了全肠外营养（TPN）治疗的大鼠与喂食普通饲料的大鼠相比，肠道形态和黏液凝胶的改变是否与鼠伤寒沙门氏菌移位的差异相关。
将27只雄性Wistar大鼠分为两组：一组接受TPN治疗14天，另一组（对照组）自由摄取标准大鼠饲料和水。将鼠伤寒沙门氏菌（5×10⁸个菌落形成单位；岐阜12142株）注入闭合的回肠肠袢。采集门静脉血（PVB）、下腔静脉血（IVCB）和肠系膜淋巴结（MLN）样本以评估细菌移位情况。制备肠袢切片，用苏木精和伊红、过碘酸-希夫（PAS）以及异硫氰酸荧光素标记的荆豆凝集素I（FITC-UEA-I）染色，用于图像分析。测量周长、黏膜厚度、绒毛面积和阳性染色的黏液面积。还进行了荧光抗体研究。
在13只对照大鼠中有1只的培养物中发现了细菌，14只TPN大鼠中有9只的培养物中发现了细菌。MLN中的细菌比PVB或IVCB中的更多。PVB、IVCB或MLN中的细菌数量没有随时间增加。TPN组的周长和绒毛面积（P<0.001）以及黏膜厚度（P<0.01）明显小于对照组。TPN组阳性染色的黏液面积明显小于对照组（PAS染色P<0.05，FITC-UEA-I染色P<0.01）。鼠伤寒沙门氏菌特异性地通过派伊尔结侵入。在所有培养阴性的样本中，细菌被黏液层捕获，只有极少数附着在上皮表面。
在TPN治疗2周后，明显的绒毛萎缩和黏液减少在鼠伤寒沙门氏菌通过大鼠派伊尔结快速移位中起重要作用。
