Romo-Figueroa María Gabriela, Vargas-Requena Claudia, Sotelo-Mundo Rogerio R, Vargas-Albores Francisco, Higuera-Ciapara Inocencio, Söderhäll Kenneth, Yepiz-Plascencia Gloria
Molecular Biology of Aquatic Organisms, DTAOA, Centro de Investigación en Alimentación y Desarrollo, A.C., P.O. Box 1735, Hermosillo, Sonora 83000, México.
Dev Comp Immunol. 2004 Jun;28(7-8):713-26. doi: 10.1016/j.dci.2003.11.008.
The hemolymph pattern-recognition beta-glucan binding protein from the white shrimp Penaeus (Litopenaeus) vannamei is also a high density lipoprotein (betaGBP-HDL) involved in innate immunity. The betaGBP-HDL full length cDNA sequence determined was 6.3 kb long, and contains a long 3'UTR region with a polyadenylation signal and a poly-A+ tail. The open reading frame is 1454 amino acids long and the N-terminal residue of the mature protein is localized in position 198 of the ORF. Comparison of the betaGBP-HDL amino acid sequence against GenBank detected only significant similarity to betaGBP from the crayfish Pacifastacus leniusculus. betaGBP-HDL is expressed in hepatopancreas, muscle, pleopods and gills, but not in hemocytes as determined by RT-PCR. We discuss the analysis of the deduced primary sequence in terms of the predicted secondary structure, glucanase-like and RGD motives relevant to its dual roles in defence and lipid transport.
南美白对虾（凡纳滨对虾）的血淋巴模式识别β-葡聚糖结合蛋白也是一种参与先天免疫的高密度脂蛋白（βGBP-HDL）。所确定的βGBP-HDL全长cDNA序列长6.3 kb，包含一个带有多聚腺苷酸化信号和多聚A+尾的长3'UTR区域。开放阅读框长1454个氨基酸，成熟蛋白的N端残基位于ORF的第198位。将βGBP-HDL氨基酸序列与GenBank进行比较，仅发现与小龙虾美洲鳌虾的βGBP有显著相似性。通过RT-PCR测定，βGBP-HDL在肝胰腺、肌肉、腹肢和鳃中表达，但在血细胞中不表达。我们根据预测的二级结构、与防御和脂质运输双重作用相关的葡聚糖酶样和RGD基序，对推导的一级序列进行了分析。
