The Queensland Department of Primary Industries and Fisheries, Animal Science, Bribie Island, Queensland, Australia.
Int J Biol Sci. 2012;8(3):328-43. doi: 10.7150/ijbs.3517. Epub 2012 Feb 9.
In crustaceans, a range of physiological processes involved in ovarian maturation occurs in organs of the cephalothorax including the hepatopancrease, mandibular and Y-organ. Additionally, reproduction is regulated by neuropeptide hormones and other proteins released from secretory sites within the eyestalk. Reproductive dysfunction in captive-reared prawns, Penaeus monodon, is believed to be due to deficiencies in these factors. In this study, we investigated the expression of gene transcripts in the cephalothorax and eyestalk from wild-caught and captive-reared animals throughout ovarian maturation using custom oligonucleotide microarray screening. We have isolated numerous transcripts that appear to be differentially expressed throughout ovarian maturation and between wild-caught and captive-reared animals. In the cephalothorax, differentially expressed genes included the 1,3-β-D-glucan-binding high-density lipoprotein, 2/3-oxoacyl-CoA thiolase and vitellogenin. In the eyestalk, these include gene transcripts that encode a protein that modulates G-protein coupled receptor activity and another that encodes an architectural transcription factor. Each may regulate the expression of reproductive neuropeptides, such as the crustacean hyperglycaemic hormone and molt-inhibiting hormone. We could not identify differentially expressed transcripts encoding known reproductive neuropeptides in the eyestalk of either wild-caught or captive-reared prawns at any ovarian maturation stage, however, this result may be attributed to low relative expression levels of these transcripts. In summary, this study provides a foundation for the study of target genes involved in regulating penaeid reproduction.
在甲壳动物中，涉及卵巢成熟的一系列生理过程发生在头胸部器官中，包括肝胰腺、下颚和 Y 器官。此外，生殖受神经肽激素和其他从眼柄分泌部位释放的蛋白质调节。养殖对虾的生殖功能障碍被认为是由于这些因素的缺乏。在这项研究中，我们使用定制的寡核苷酸微阵列筛选，研究了野生和养殖对虾头胸部和眼柄在整个卵巢成熟过程中基因转录本的表达。我们已经分离出许多似乎在整个卵巢成熟过程中以及在野生和养殖动物之间差异表达的转录本。在头胸部，差异表达的基因包括 1,3-β-D-葡聚糖结合高密度脂蛋白、2/3-氧代酰基辅酶 A 硫解酶和卵黄原蛋白。在眼柄中，这些包括编码调节 G 蛋白偶联受体活性的蛋白质的基因转录本和另一种编码结构转录因子的基因转录本。它们每个都可能调节生殖神经肽的表达，例如甲壳动物高血糖激素和蜕皮抑制激素。然而，我们无法在任何卵巢成熟阶段的野生或养殖对虾的眼柄中识别出差异表达的编码已知生殖神经肽的转录本，但这一结果可能归因于这些转录本的相对表达水平较低。总之，这项研究为研究参与调节对虾生殖的靶基因提供了基础。
