Wang J, de Boer J, de Groot K
IsoTis S.A., Prof. Bronkhorstlaan 10-D, 3723 MB Bilthoven, The Netherlands.
J Dent Res. 2004 Apr;83(4):296-301. doi: 10.1177/154405910408300405.
Electrolytically deposited carbonate apatite coating demonstrates higher strength but weaker support for bone marrow stromal cell attachment than do biomimetically deposited coatings. It is hypothesized that the incorporation of chitosan will increase the biocompatibility of electrolytic coating while maintaining its original strength. To verify this hypothesis, we formed a hybrid calcium phosphate/chitosan coating through electrodeposition. We found that the incorporation of chitosan influenced calcium phosphate formation and crystallization. Moreover, coating thickness and surface roughness decreased with increasing chitosan concentration. Hybrid coating exhibited an increased dissolution rate in both acidic and neutral simulated physiologic solution, whereas no significant difference on adhesive strength was found between the hybrid and original coatings (P > 0.05). Most importantly, the calcium phosphate/chitosan coating proved to be a more favorable surface for goat bone marrow stromal cell attachment than an unincorporated coating (P < 0.01). Considering its economic and simple production, a hybrid calcium phosphate/chitosan coating is thought to be an attractive candidate for future applications.
与仿生沉积涂层相比，电解沉积碳酸磷灰石涂层显示出更高的强度，但对骨髓基质细胞附着的支持较弱。据推测，壳聚糖的加入将提高电解涂层的生物相容性，同时保持其原有强度。为了验证这一假设，我们通过电沉积形成了一种磷酸钙/壳聚糖混合涂层。我们发现壳聚糖的加入影响了磷酸钙的形成和结晶。此外，涂层厚度和表面粗糙度随壳聚糖浓度的增加而降低。混合涂层在酸性和中性模拟生理溶液中的溶解速率均有所增加，而混合涂层与原始涂层之间的粘附强度没有显著差异（P>0.05）。最重要的是，磷酸钙/壳聚糖涂层被证明是比未添加涂层更有利于山羊骨髓基质细胞附着的表面（P<0.01）。考虑到其经济且简单的生产方式，磷酸钙/壳聚糖混合涂层被认为是未来应用中一个有吸引力的候选材料。
