Chimma Phattamawan, Chirathaworn Chintana, Bhattarakosol Parvapan
Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Intervirology. 2004;47(1):14-8. doi: 10.1159/000076637.
To study herpes simplex virus (HSV) growth in human T lymphocytes (Jurkat cells) with and without phytohemagglutinin (PHA) activation and to investigate the possible mechanism of viral growth.
The levels of HSV-1 production and adsorption were determined by plaque titration assay. The number of HSV-1-infected cells was assayed using flow cytometry. The expression of herpesvirus entry mediator A (HveA) mRNA was detected by RT-PCR.
HSV-1 production as well as the number of HSV-1-infected Jurkat cells were enhanced after the cells were activated by PHA. Moreover, the amount of viral entry was demonstrated to increase in PHA-activated cells. An increase in HveA mRNA was observed in PHA-activated Jurkat cells.
We found that HSV-1 can replicate in human T lymphocytes, and the replication was increased following PHA activation. This finding may be due to an increase in viral entry via HveA receptor.
研究单纯疱疹病毒（HSV）在有或无植物血凝素（PHA）激活的人T淋巴细胞（Jurkat细胞）中的生长情况，并探讨病毒生长的可能机制。
通过噬斑滴定法测定HSV-1的产生水平和吸附情况。使用流式细胞术检测HSV-1感染细胞的数量。通过逆转录聚合酶链反应（RT-PCR）检测疱疹病毒进入介质A（HveA）mRNA的表达。
PHA激活细胞后，HSV-1的产生以及HSV-1感染的Jurkat细胞数量均增加。此外，在PHA激活的细胞中，病毒进入量也增加。在PHA激活的Jurkat细胞中观察到HveA mRNA增加。
我们发现HSV-1可在人T淋巴细胞中复制，且PHA激活后复制增加。这一发现可能是由于通过HveA受体的病毒进入增加所致。
