用乙醛酸2,4-二硝基苯腙通过色谱-分光光度法和差分脉冲极谱法测定乙醛酸

Use of 2,4-dinitrophenylhydrazone of glyoxylic acid for the determination of glyoxylic acid by the chromatographic-spectrophotometric method and by differential pulse polarography.

作者信息

Masłowska J, Duda J, Witusik A

机构信息

Institute of General Food Chemistry, Technical University of Łódź, 4/10 B. Stefanowskiego St., PL-90924, Łódź, Poland.

出版信息

Anal Bioanal Chem. 1996 May;355(2):154-6. doi: 10.1007/s0021663550154.

DOI:10.1007/s0021663550154

PMID:15045440

Abstract

Two different methods (one based on chromatography and spectrophotometry and the other on polarography) have been developed for the determination of glyoxylic acid in the form of a derivative with 2,4-dinitrophenylhydrazine (DNPH. GA). The TLC method allows the separation of two DNPH. GA isomers ("trans" and "cis"). Spectrophotometric measurements of the eluents of the separated compounds (lambda=360 nm) allow the determination of GA within the range from 4 to 30 microg. Using differential pulse polarography, the conditions of DNPH. GA formation were examined. The reduction peak of this derivative (E(P)=-0.430 V), observed by dpp, was used to develop a polarographic determination of GA within the concentration range from 1.10(-4) to 7.10(-4) mol/l.

摘要

已开发出两种不同的方法（一种基于色谱法和分光光度法，另一种基于极谱法）来测定以2,4-二硝基苯肼衍生物（DNPH.GA）形式存在的乙醛酸。薄层色谱法可分离两种DNPH.GA异构体（“反式”和“顺式”）。对分离出的化合物洗脱液进行分光光度测量（λ=360nm），可测定4至30微克范围内的乙醛酸。使用差分脉冲极谱法，研究了DNPH.GA的形成条件。通过差分脉冲极谱法观察到该衍生物的还原峰（E(P)= -0.430V），用于建立浓度范围为1.10(-4)至7.10(-4)mol/l的乙醛酸极谱测定法。