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一种用于测定乙醛酸合成混合物中乙醛酸的简易分光光度法。

A Simple Spectrophotometric Method for Determination of Glyoxylic Acid in Its Synthesis Mixture.

作者信息

Abdulwahed Mazhar, Mamoly Lamia, Bosnali Wael

机构信息

Damascus University, Faculty of Sciences, Damascus, Syria.

出版信息

Int J Anal Chem. 2020 Mar 19;2020:5417549. doi: 10.1155/2020/5417549. eCollection 2020.

DOI:10.1155/2020/5417549
PMID:32308686
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7155763/
Abstract

A new simple and reliable spectrophotometric method is described to determine glyoxylic acid in its synthesis reaction mixture containing oxalic acid, glycolic acid, acetic acid, glyoxal, and ethylene glycol by means of a modified Hopkins-Cole reaction between glyoxylic acid and tryptophan in presence of ferric chloride and concentrated sulphuric acid. The linear range of glyoxylic acid concentration is 0-0.028 M. The limits of detection (LOD) and quantitation (LOQ) are 0.0019 M and 0.00577 M, respectively. The LOD, LOQ, standard deviation, relative standard deviation, and recovery ratio of the proposed method are comparable with a selected HPLC reference method. Both methods displayed same precision and credibility. Reaction stoichiometry between tryptophan and glyoxylic acid is assumed to be 2 : 3. Reaction mechanism has been postulated based on identified molar ratios of reactants. Glyoxal gave a negative test with tryptophan although it is a dialdehyde.

摘要

本文描述了一种新的简单可靠的分光光度法，用于测定合成反应混合物中的乙醛酸，该混合物中含有草酸、乙醇酸、乙酸、乙二醛和乙二醇。方法是利用乙醛酸与色氨酸在氯化铁和浓硫酸存在下发生改良的霍普金斯-科尔反应。乙醛酸浓度的线性范围为0-0.028M。检测限（LOD）和定量限（LOQ）分别为0.0019M和0.00577M。该方法的LOD、LOQ、标准差、相对标准差和回收率与选定的HPLC参考方法相当。两种方法具有相同的精密度和可信度。色氨酸与乙醛酸之间的反应化学计量比假定为2∶3。基于确定的反应物摩尔比推测了反应机理。虽然乙二醛是二醛，但它与色氨酸反应呈阴性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9f/7155763/54b84ad888c9/IJAC2020-5417549.010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9f/7155763/a3cecf25959f/IJAC2020-5417549.001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9f/7155763/a3cecf25959f/IJAC2020-5417549.001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9f/7155763/53bdb40388c0/IJAC2020-5417549.004.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9f/7155763/6411b5a5c45f/IJAC2020-5417549.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9f/7155763/0983dc2ddd6f/IJAC2020-5417549.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9f/7155763/13df9f2676f3/IJAC2020-5417549.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9f/7155763/061f00eed46a/IJAC2020-5417549.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9f/7155763/54b84ad888c9/IJAC2020-5417549.010.jpg

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本文引用的文献

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Determination of glyoxylic acid in urine by liquid chromatography with fluorescence detection, using a novel derivatization procedure based on the Petasis reaction.采用基于 Petasis 反应的新型衍生化程序，通过荧光检测的液相色谱法测定尿液中的乙醛酸。
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