斐济病病毒基因组片段5、6、8和10的分子分析

Molecular analysis of Fiji disease virus genome segments 5, 6, 8 and 10.

作者信息

McQualter R B, Burns P, Smith G R, Dale J L, Harding R M

机构信息

Plant Biotechnology Program, Science Research Centre, Queensland University of Technology, Brisbane, Australia.

出版信息

Arch Virol. 2004 Apr;149(4):713-21. doi: 10.1007/s00705-003-0243-1. Epub 2003 Nov 26.

DOI:10.1007/s00705-003-0243-1

PMID:15045559

Abstract

The complete sequences of Fiji disease virus (FDV) genome segments 5 (S5), S6, S8 and S10 were obtained and comprised 3150 nt, 2831 nt, 1959 nt and 1819 nt, respectively. Each segment contained a single ORF which encoded putative proteins of 115 kDa, 97 kDa, 69 kDa and 63.0 kDa, respectively. The putative amino acid sequences encoded by S5 and S6 contained putative leucine zipper motifs while FDV S5 and S8 each contained an ATP-GTP-binding motif. At the amino acid level, FDV S5, S6, S8 and S10 showed most similarity to the corresponding segments of Rice black-streaked dwarf virus. Based on sequence similarities, it is predicted that FDV S8 encodes a minor core protein, while FDV S10 encodes an outer capsid protein. The evolutionary relationships of FDV to other reoviruses are discussed.

摘要

获得了斐济病病毒（FDV）基因组片段5（S5）、S6、S8和S10的完整序列，分别为3150个核苷酸、2831个核苷酸、1959个核苷酸和1819个核苷酸。每个片段都包含一个单一的开放阅读框，分别编码推定的115 kDa、97 kDa、69 kDa和63.0 kDa的蛋白质。由S5和S6编码的推定氨基酸序列包含推定的亮氨酸拉链基序，而FDV S5和S8各自包含一个ATP - GTP结合基序。在氨基酸水平上，FDV S5、S6、S8和S10与水稻黑条矮缩病毒的相应片段显示出最大的相似性。基于序列相似性，预测FDV S8编码一种次要核心蛋白，而FDV S10编码一种外衣壳蛋白。讨论了FDV与其他呼肠孤病毒的进化关系。