植物斐济病毒第5节段中部分重叠3'开放阅读框的编码潜力分析。
Analysis of the coding potential of the partially overlapping 3' ORF in segment 5 of the plant fijiviruses.
作者信息
Firth Andrew E, Atkins John F
机构信息
BioSciences Institute, University College Cork, Cork, Ireland.
出版信息
Virol J. 2009 Mar 17;6:32. doi: 10.1186/1743-422X-6-32.
The plant-infecting members of the genus Fijivirus (family Reoviridae) have linear dsRNA genomes divided into 10 segments, two of which contain two substantial and non-overlapping ORFs, while the remaining eight are apparently monocistronic. However, one of these - namely segment 5 - contains a second long ORF (approximately 200+ codons) that overlaps the 3' end of the major ORF (approximately 920-940 codons) in the +1 reading frame. In this report, we use bioinformatic techniques to analyze the pattern of base variations across an alignment of fijivirus segment 5 sequences, and show that this 3' ORF has a strong coding signature. Possible translation mechanisms for this unusually positioned ORF are discussed.
斐济病毒属（呼肠孤病毒科）的植物感染成员具有线性双链RNA基因组，分为10个片段，其中两个片段包含两个较大且不重叠的开放阅读框（ORF），而其余八个片段显然是单顺反子的。然而，其中一个片段——即片段5——包含第二个长开放阅读框（约200多个密码子），它在+1阅读框中与主要开放阅读框（约920 - 940个密码子）的3'端重叠。在本报告中，我们使用生物信息学技术分析了斐济病毒片段5序列比对中的碱基变异模式，并表明这个3'端开放阅读框具有很强的编码特征。我们还讨论了这个位置异常的开放阅读框可能的翻译机制。
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