Collins Matthew D, Lundström Torbjörn, Welinder-Olsson Christina, Hansson Ingrid, Wattle Ove, Hudson Roger A, Falsen Enevold
School of Food Biosciences, University of Reading, Reading, UK.
Syst Appl Microbiol. 2004 Mar;27(2):146-50. doi: 10.1078/072320204322881754.
Phenotypic and phylogenetic studies were performed on four unidentified Gram-positive staining, catalase-negative, alpha-hemolytic Streptococcus-like organisms recovered from the teeth of horses. SDS PAGE analysis of whole-cell proteins and comparative 16S rRNA gene sequencing demonstrated the four strains were highly related to each other but that they did not correspond to any recognised species of the genus Streptococcus. Phylogenetic analysis based on 16S rRNA gene sequences showed the unidentified organisms form a hitherto unknown sub-line within the Streptococcus genus, displaying a close affinity with Streptococcus mutans, Streptococcus ferus and related organisms. Sequence divergence values of > 5% with these and other reference streptococcal species however demonstrated the organisms from equine sources represent a novel species. Based on the phenotypic distinctiveness of the new bacterium and molecular chemical and molecular genetic evidence, it is proposed that the unknown species be classified as Streptococcus devriesei sp. nov. The type strain of Streptococcus devriesei is CCUG 47155T (= CIP 107809T).
对从马牙齿中分离出的4株未鉴定的革兰氏阳性、过氧化氢酶阴性、α-溶血的类链球菌进行了表型和系统发育研究。全细胞蛋白的SDS-PAGE分析和16S rRNA基因序列比较显示，这4株菌株彼此高度相关，但它们与链球菌属的任何已知种均不对应。基于16S rRNA基因序列的系统发育分析表明，这些未鉴定的微生物在链球菌属内形成了一个迄今未知的亚系，与变形链球菌、野链球菌及相关微生物有密切的亲缘关系。然而，与这些及其他参考链球菌种的序列差异值>5%，表明来自马源的微生物代表一个新种。基于新细菌的表型特征以及分子化学和分子遗传学证据，建议将该未知种分类为德弗里斯链球菌（Streptococcus devriesei）新种。德弗里斯链球菌的模式菌株是CCUG 47155T（=CIP 107809T）。
