Milinovich Gabriel J, Burrell Paul C, Pollitt Christopher C, Bouvet Anne, Trott Darren J
Australian Equine Laminitis Research Unit, School of Veterinary Science, The University of Queensland, Brisbane, Queensland 4072, Australia.
Int J Syst Evol Microbiol. 2008 Jan;58(Pt 1):262-6. doi: 10.1099/ijs.0.65063-0.
Four Gram-positive, catalase-negative, coccoid-shaped isolates were obtained from the caecum and rectum of horses with oligofructose-induced equine laminitis. Phenotypic and phylogenetic studies were performed on these isolates. Initial biochemical profiling assigned two of the isolates to Streptococcus bovis. The other two isolates, however, could not be assigned conclusively on the basis of their biochemical profiles. Gene sequence analysis demonstrated that the four new isolates were related most closely to Streptococcus suis based on the 16S rRNA gene and to Streptococcus orisratti based on the manganese-dependent superoxide dismutase gene (sodA). Sequence divergence values from recognized Streptococcus species based on these two genes were >3 and >13%, respectively, for all four isolates. Phylogenetic and phenotypic analyses demonstrated that the four isolates formed two distinct clonal groups that are suggested to represent two novel species of the genus Streptococcus. The names proposed for these organisms are Streptococcus henryi sp. nov. (type strain 126(T) =ATCC BAA-1484(T) =DSM 19005(T)) and Streptococcus caballi sp. nov. (type strain 151(T) =ATCC BAA-1485(T) =DSM 19004(T)).
从患有低聚果糖诱导的马属动物蹄叶炎的马的盲肠和直肠中获得了 4 株革兰氏阳性、过氧化氢酶阴性、球形分离株。对这些分离株进行了表型和系统发育研究。初步生化分析将其中 2 株分离株归为牛链球菌。然而，另外 2 株分离株根据其生化谱无法明确归类。基因序列分析表明，基于 16S rRNA 基因，这 4 株新分离株与猪链球菌关系最为密切；基于锰依赖性超氧化物歧化酶基因（sodA），与鼠链球菌关系最为密切。基于这两个基因，所有 4 株分离株与已确认的链球菌属物种的序列差异值分别>3%和>13%。系统发育和表型分析表明，这 4 株分离株形成了两个不同的克隆群，提示它们代表链球菌属的两个新物种。为这些菌株提议的名称分别是亨利链球菌新种（模式菌株 126(T)=ATCC BAA - 1484(T)=DSM 19005(T)）和马链球菌新种（模式菌株 151(T)=ATCC BAA - 1485(T)=DSM 19004(T)）。
