Gallová L, Kubala L, Cíz M, Lojek A
Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic.
Physiol Res. 2004;53(2):199-208.
Cytokines play a major role in the control of inflammatory responses, participate in the regulation of blood phagocyte activities and as such are used for immunomodulatory therapy. In the present study, the influence of IL-10 on human blood phagocyte activity in the presence/absence of IL-6, IL-8 and TNF-alpha was tested in vitro. Our research analyzed the effects of cytokines on the production of reactive oxygen species measured by chemiluminescence and flow cytometry, and on the expression of surface molecules (CD11b, CD15, CD62L, CD31) measured by flow cytometry. IL-10 had no inhibitory effect on reactive oxygen species production and the expression of any examined adhesion molecule by resting or stimulated blood phagocytes within 3 h of incubation. Conversely, TNF-alpha, IL-6, and IL-8 increased reactive oxygen species production and the expression of CD11b and CD15 on both neutrophils and monocytes and decreased the expression of CD62L. These priming effects of the tested pro-inflammatory cytokines were not affected by IL-10. The obtained results suggest that IL-10 does not directly control blood phagocyte activation. These results also provide better information about the contribution of IL-6, IL-8 and TNF-alpha to the regulation of blood phagocyte-mediated inflammatory processes.
细胞因子在炎症反应的控制中起主要作用，参与血液吞噬细胞活性的调节，因此被用于免疫调节治疗。在本研究中，体外测试了白细胞介素-10（IL-10）在存在/不存在白细胞介素-6（IL-6）、白细胞介素-8（IL-8）和肿瘤坏死因子-α（TNF-α）的情况下对人血液吞噬细胞活性的影响。我们的研究分析了细胞因子对通过化学发光和流式细胞术测量的活性氧产生的影响，以及对通过流式细胞术测量的表面分子（CD11b、CD15、CD62L、CD31）表达的影响。在孵育3小时内，IL-10对静息或受刺激的血液吞噬细胞产生活性氧以及任何检测的黏附分子的表达均无抑制作用。相反，TNF-α、IL-6和IL-8增加了中性粒细胞和单核细胞中活性氧的产生以及CD11b和CD15的表达，并降低了CD62L的表达。所测试的促炎细胞因子的这些启动作用不受IL-10的影响。获得的结果表明，IL-10不直接控制血液吞噬细胞的激活。这些结果还提供了关于IL-6、IL-8和TNF-α对血液吞噬细胞介导的炎症过程调节作用的更好信息。
