Oelke Mathias, Schneck Jonathan P
Department of Pathology and Medicine, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
Clin Immunol. 2004 Mar;110(3):243-51. doi: 10.1016/j.clim.2003.11.014.
Recent advances in molecular medicine have shown that soluble MHC-multimers can be valuable tools for both the stimulation of as well as the analysis of antigen-specific T cells in vitro. In this review, we describe the use of dimeric major histocompatibility complexes, HLA-Ig, to visualize antigen-specific T cells as well as their potential to stimulate immune responses as part of an artificial antigen-presenting cell (aAPC). The use of HLA-Ig-based aAPC represents an exciting new approach to generate antigen-specific CTL for adoptive immunotherapy that helps to overcome many of the obstacles associated with limitations in current approaches to adoptive immunotherapy.
分子医学的最新进展表明，可溶性MHC多聚体在体外刺激和分析抗原特异性T细胞方面可成为有价值的工具。在本综述中，我们描述了二聚体主要组织相容性复合体HLA-Ig用于可视化抗原特异性T细胞的用途，以及其作为人工抗原呈递细胞（aAPC）的一部分刺激免疫反应的潜力。基于HLA-Ig的aAPC的应用代表了一种令人兴奋的新方法，可用于生成用于过继性免疫治疗的抗原特异性CTL，有助于克服与当前过继性免疫治疗方法局限性相关的许多障碍。
