健康人全血中一氧化氮（NO）代谢对其红细胞血红蛋白氧合作用的依赖性。
Dependence of the metabolism of nitric oxide (NO) in healthy human whole blood on the oxygenation of its red cell haemoglobin.
作者信息
Wennmalm A, Benthin G, Petersson A S
机构信息
Department of Clinical Physiology, Gothenburg University, Sahlgrenska Hospital, Sweden.
出版信息
Br J Pharmacol. 1992 Jul;106(3):507-8. doi: 10.1111/j.1476-5381.1992.tb14365.x.
Abstract
Plasma or whole venous or arterialized blood from healthy human donors was incubated with NO (50-300 microM), and the resulting formation of methaemoglobin (MetHb), nitrosyl haemoglobin (HbNO), and plasma nitrite and nitrate were measured. In plasma, NO was converted to nitrite and nitrate in a ratio of 5:1. In arterial blood (O2 sat. 94-99%) NO was almost quantitatively converted to nitrate and MetHb. No nitrite was detected and HbNO formation was low. In venous blood (O2 sat. 36-85%) more HbNO and less nitrate was formed, in comparison to arterialized blood. We propose that NO liberated from endothelium of conductance and resistance vessels is taken up by red blood cells and inactivated by HbO2 via stoichiometric conversion to MetHb and nitrate.
摘要
将健康人体供体的血浆或全静脉血或动脉化血液与一氧化氮（NO，50 - 300微摩尔）一起孵育，然后测量由此产生的高铁血红蛋白（MetHb）、亚硝基血红蛋白（HbNO）以及血浆亚硝酸盐和硝酸盐的形成情况。在血浆中，NO以5:1的比例转化为亚硝酸盐和硝酸盐。在动脉血（氧饱和度94 - 99%）中，NO几乎定量地转化为硝酸盐和MetHb。未检测到亚硝酸盐，且HbNO的形成量较低。与动脉化血液相比，在静脉血（氧饱和度36 - 85%）中形成了更多的HbNO和更少的硝酸盐。我们提出，从传导血管和阻力血管内皮释放的NO被红细胞摄取，并通过化学计量转化为MetHb和硝酸盐而被HbO₂灭活。
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