Mittova Valentina, Guy Micha, Tal Moshe, Volokita Micha
Albert Katz Department of Dryland Biotechnologies, Jacob Blaustein Institute for Desert Research, Ben-Gurion University of the Negev, Sede Boqer Campus 84990, Israel.
J Exp Bot. 2004 May;55(399):1105-13. doi: 10.1093/jxb/erh113. Epub 2004 Mar 26.
The effect of salinity on the antioxidative system of root mitochondria and peroxisomes of a cultivated tomato Lycopersicon esculentum (Lem) and its wild salt-tolerant related species L. pennellii (Lpa) was studied. Salt stress induced oxidative stress in Lem mitochondria, as indicated by the increased levels of lipid peroxidation and H(2)O(2). These changes were associated with decreased activities of superoxide dismutase (SOD) and guaiacol peroxidases (POD) and contents of ascorbate (ASC) and glutathione (GSH). By contrast, in mitochondria of salt-treated Lpa plants both H(2)O(2) and lipid peroxidation levels decreased while the levels of ASC and GSH and activities of SOD, several isoforms of ascorbate peroxidase (APX), and POD increased. Similarly to mitochondria, peroxisomes isolated from roots of salt-treated Lpa plants exhibited also decreased levels of lipid peroxidation and H(2)O(2) and increased SOD, ascorbate peroxidase (APX), and catalase (CAT) activities. In spite of the fact that salt stress decreased activities of antioxidant enzymes in Lem peroxisome, oxidative stress was not evident in these organelles.
研究了盐度对栽培番茄（Lycopersicon esculentum (Lem)）及其野生耐盐近缘种彭奈利番茄（L. pennellii (Lpa)）根系线粒体和过氧化物酶体抗氧化系统的影响。盐胁迫诱导了Lem线粒体中的氧化应激，脂质过氧化水平和H₂O₂含量增加表明了这一点。这些变化与超氧化物歧化酶（SOD）和愈创木酚过氧化物酶（POD）活性降低以及抗坏血酸（ASC）和谷胱甘肽（GSH）含量降低有关。相比之下，在盐处理的Lpa植株线粒体中，H₂O₂和脂质过氧化水平均降低，而ASC和GSH水平以及SOD、几种抗坏血酸过氧化物酶（APX）同工型和POD的活性增加。与线粒体类似，从盐处理的Lpa植株根系分离的过氧化物酶体也表现出脂质过氧化和H₂O₂水平降低，以及SOD、抗坏血酸过氧化物酶（APX）和过氧化氢酶（CAT）活性增加。尽管盐胁迫降低了Lem过氧化物酶体中抗氧化酶的活性，但这些细胞器中并未明显出现氧化应激。
