Tokunaga K, Ishikawa K
Department of Neurology, Nihon University School of Medicine, Tokyo, Japan.
Brain Res. 1992 Mar 13;575(1):164-6. doi: 10.1016/0006-8993(92)90439-g.
Wistar rats were implanted with a probe for brain microdialysis in the striatum, and measured for two major dopamine metabolites, 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA). Intraperitoneal chlorpromazine (5 mg/kg) increased the concentrations of both metabolites in the dialysate when the determinations were carried out before 90 min after the administration. An apparent difference between the declines in concentrations of DOPAC and HVA was observed. Although the concentration of HVA maintained higher levels for over 6 h after the administration, DOPAC underwent a decrease in its concentration after the maximum level, which occurred at 90 min after the administration. These results are discussed in terms of the different distributions of two enzymes related to the metabolism of dopamine, catechol-O-methyltransferase and monoamine oxidase.
将Wistar大鼠的纹状体植入脑微透析探针，并测量两种主要的多巴胺代谢产物，即3,4-二羟基苯乙酸（DOPAC）和高香草酸（HVA）。在给药后90分钟之前进行测定时，腹腔注射氯丙嗪（5毫克/千克）会增加透析液中两种代谢产物的浓度。观察到DOPAC和HVA浓度下降之间存在明显差异。尽管给药后HVA的浓度在6小时以上保持较高水平，但DOPAC在给药后90分钟达到最高水平后其浓度出现下降。根据与多巴胺代谢相关的两种酶，即儿茶酚-O-甲基转移酶和单胺氧化酶的不同分布来讨论这些结果。
