Purbhoo Marco A, Irvine Darrell J, Huppa Johannes B, Davis Mark M
Department of Microbiology and Immunology and the Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California 94305, USA.
Nat Immunol. 2004 May;5(5):524-30. doi: 10.1038/ni1058. Epub 2004 Mar 28.
A notable feature of T lymphocyte recognition on other cell surfaces is the formation of a stable mature immunological synapse. Here we use a single-molecule labeling method to directly measure the number of ligands a cytotoxic T cell engages and track the consequences of that interaction by three-dimensional video microscopy. Like helper T cells, cytotoxic T cells were able to detect even a single foreign antigen but required about ten complexes of peptide-major histocompatibility complex (pMHC) to achieve full calcium increase and to form a mature synapse. Thus, cytotoxic T cells and helper T cells are more uniform in their antigen sensitivities than previously thought. Furthermore, only three pMHC complexes were required for killing, showing that stable synapse formation and complete signaling are not required for cytotoxicity.
T淋巴细胞在其他细胞表面识别的一个显著特征是形成稳定的成熟免疫突触。在这里，我们使用单分子标记方法直接测量细胞毒性T细胞接触的配体数量，并通过三维视频显微镜追踪这种相互作用的结果。与辅助性T细胞一样，细胞毒性T细胞即使只能检测到单一的外来抗原，但也需要大约十个肽-主要组织相容性复合体（pMHC）复合物才能实现完全的钙增加并形成成熟的突触。因此，细胞毒性T细胞和辅助性T细胞在抗原敏感性方面比之前认为的更加一致。此外，杀伤只需三个pMHC复合物，这表明细胞毒性并不需要稳定的突触形成和完整的信号传导。
