一项双标记研究表明，幼体虎螈视网膜中所有血清素样免疫反应性无长突细胞均表达γ-氨基丁酸样免疫反应性。

A double-label study demonstrating that all serotonin-like immunoreactive amacrine cells in the larval tiger salamander retina express GABA-like immunoreactivity.

作者信息

Watt C B

机构信息

Alice R. McPherson Laboratory of Retina Research, Center for Biotechnology, Baylor College of Medicine, The Woodlands, TX 77381.

出版信息

Brain Res. 1992 Jun 26;583(1-2):336-9. doi: 10.1016/s0006-8993(10)80046-0.

DOI:10.1016/s0006-8993(10)80046-0

PMID:1504841

Abstract

A previous study localized serotonin-like immunoreactivity to amacrine cell populations in the larval tiger salamander retina. The present double-label immunocytochemical analysis of the tiger salamander retina was performed to determine if gamma-aminobutyric acid (GABA)-like immunoreactivity is expressed by serotonin-immunoreactive amacrine cells. More than 3,000 serotonin-amacrine cells were observed in double-label preparations, and all were found to express GABA-like immunoreactivity. This finding extends previous studies of serotonin-GABA coexistence in the retina by providing the first report of the co-localization of endogenous serotonin and GABA-like compounds in a retinal neuron.

摘要

先前的一项研究将5-羟色胺样免疫反应定位到了虎螈幼体视网膜中的无长突细胞群。本研究对虎螈视网膜进行了双标记免疫细胞化学分析，以确定5-羟色胺免疫反应性无长突细胞是否表达γ-氨基丁酸（GABA）样免疫反应性。在双标记制剂中观察到了3000多个5-羟色胺无长突细胞，并且发现它们均表达GABA样免疫反应性。这一发现通过首次报道内源性5-羟色胺和GABA样化合物在视网膜神经元中的共定位，扩展了先前关于视网膜中5-羟色胺与GABA共存的研究。