Quantitative analyses of the coexistence of gamma-aminobutyric acid in substance P-amacrine cells of the larval tiger salamander retina.

The present study was performed as part of a systematic examination of gamma-aminobutyric acid's (GABA) coexistence with other classical transmitters and neuropeptides in neuronal populations of the larval tiger salamander retina. Substance P immunocytochemistry was combined with either GABA immunocytochemistry or autoradiography of high-affinity GABA uptake to examine for the presence of GABA in substance P-amacrine cells of the larval tiger salamander retina. Double-label analyses revealed two populations of substance P-amacrine cells that express both markers of GABA activity. One population was situated in the innermost cell row of the inner nuclear layer, while the other population was located in the ganglion cell layer. In both cases, these double-labelled cells accounted for approximately 10% of substance P-amacrine cells in their respective layers. The present study demonstrates, therefore, that substance P-amacrine cells in the larval tiger salamander retina can be categorized on the basis of their coexisting/non-coexisting relationships with GABA and suggests a possible functional diversity in the population of substance P-amacrine cells.