Petibois Cyril, Desbat Bernard, Déléris Gérard
Université Victor Segalen Bordeaux 2, INSERM U577, Groupe de Chimie Bio-Organique, 146 rue Léo Saignat, F33076 Bordeaux, France.
Biopolymers. 2004 Apr 15;73(6):696-704. doi: 10.1002/bip.20002.
For determining exercise-induced changes in plasma molecular species, we investigated the feasibility of using two-dimensional correlation spectra (2D-COS) on 21 series of plasma samples obtained from progressive exercise tests. Intensities of 2D synchronous and asynchronous correlation peaks were compared to concentration evolutions of glucose, lactate, triglycerides (TG), glycerol, fatty acyl moieties, amino acids, proteins, and albumin [determined from plasma Fourier transform infrared (FTIR) spectra] and to performance and training levels of athletes. Synchronous 2D-COS allowed us to determine the linear relationship between protein and fatty acid concentration evolutions as exercise intensity increased (nu1-nu2; 2959 vs 1656 and 1543 cm(-1)). Asynchronous 2D-COS allowed differentiation of fibrinolysis level in subjects during intense exercise, as well as parameters of fatty acid metabolism specifically related either to performance or to training levels. Furthermore, unexpected correlated evolutions of molecular species were highlighted by this method, showing that 2D spectrometry may also be used for experimental investigations on human physiology. This study has demonstrated that 2D-COS may be used for the treatment of complex biological samples, such as plasma.
为了确定运动引起的血浆分子种类变化，我们研究了对从渐进性运动测试中获得的21组血浆样本使用二维相关光谱（2D-COS）的可行性。将二维同步和异步相关峰的强度与葡萄糖、乳酸、甘油三酯（TG）、甘油、脂肪酸部分、氨基酸、蛋白质和白蛋白的浓度变化进行比较[由血浆傅里叶变换红外（FTIR）光谱测定]，并与运动员的表现和训练水平进行比较。同步二维相关光谱使我们能够确定随着运动强度增加蛋白质和脂肪酸浓度变化之间的线性关系（ν1-ν2；2959与1656和1543 cm(-1)）。异步二维相关光谱能够区分剧烈运动期间受试者的纤溶水平，以及与表现或训练水平特别相关的脂肪酸代谢参数。此外，该方法突出了分子种类意外的相关变化，表明二维光谱法也可用于人体生理学的实验研究。本研究表明二维相关光谱可用于处理复杂的生物样本，如血浆。
