Moriki Toshiaki, Takahashi Tamotsu, Ueta Shousuke, Mitani Miko, Ichien Miho
Department of Clinical Laboratory, Kochi Medical School Hospital, Nankoku, Kochi, Japan.
Diagn Cytopathol. 2004 Apr;30(4):251-6. doi: 10.1002/dc.20007.
Although fine-needle aspiration cytology is a routine procedure for the diagnosis of breast carcinoma, cytologic specimens have rarely been used for evaluation of hormone receptor status and HER2/neu overexpression. In order to compare the biological markers on cytology and on histology, routinely fixed smears of 110 primary breast carcinomas were immunostained for estrogen receptor (ER), progesterone receptor (PgR), and HER2/neu by automated immunostainer and the results were compared with the corresponding histologic sections. ER was expressed in 76 of 110 (69%) cases and PgR was expressed in 51 of 110 (46%). Overexpression of HER2/neu was observed in 30 of 110 (27%) cases. Concordance between cytology and histology was 98% for ER, 95% for PgR, and 100% for HER2/neu. There was no false positive result on smears. Diagnostic pitfalls in determination of hormone receptor status on smears included intratumoral heterogeneity and presence of mucin.
尽管细针穿刺细胞学检查是诊断乳腺癌的常规方法，但细胞学标本很少用于评估激素受体状态和HER2/neu过表达情况。为了比较细胞学和组织学上的生物标志物，对110例原发性乳腺癌的常规固定涂片进行雌激素受体（ER）、孕激素受体（PgR）和HER2/neu的自动免疫染色，并将结果与相应的组织学切片进行比较。110例中有76例（69%）表达ER，110例中有51例（46%）表达PgR。110例中有30例（27%）观察到HER2/neu过表达。ER细胞学与组织学的一致性为98%，PgR为95%，HER2/neu为100%。涂片无假阳性结果。涂片上激素受体状态判定的诊断陷阱包括肿瘤内异质性和黏液的存在。
