环氧化酶-2在人肾细胞癌中的表达及其与肿瘤进展的关系。

Cyclooxygenase-2 expression and relationship to tumour progression in human renal cell carcinoma.

作者信息

Hashimoto Y, Kondo Y, Kimura G, Matsuzawa I, Sato S, Ishizaki M, Imura N, Akimoto M, Hara S

机构信息

Department of Urology, Central Institute for Electron Microscopic Research, Kitasato University, Tokyo, Japan.

出版信息

Histopathology. 2004 Apr;44(4):353-9. doi: 10.1111/j.1365-2559.2004.01853.x.

DOI:10.1111/j.1365-2559.2004.01853.x

PMID:15049901

Abstract

AIMS

Cyclooxygenase (COX), which catalyses the synthesis of prostaglandins from arachidonic acid, has two isoforms; COX-1 and COX-2. There is ample evidence to suggest an important role for COX-2 in cancer. The aim of this study was to evaluate the clinical significance of COX-2 expression and its localization in the development and progression of human renal cell carcinoma (RCC).

METHODS AND RESULTS

The expression and localization of COX-2 were evaluated in human RCC tissues from 75 patients by immunohistochemistry. Immunoreactive COX-2 protein was observed in all cases of RCC, and the levels of COX-2 expression were correlated with tumour grade and pathological stage. Expression of COX-2 was higher in the granular cell subtype than in the clear cell subtype of RCC. Immunoelectron microscopy revealed that COX-2 was expressed in the nuclear membrane, rough endoplasmic reticulum, Golgi complex and mitochondrial membrane of RCC cells.

CONCLUSION

COX-2 overexpression within these intracellular organelles in RCC may be associated with renal cell carcinogenesis and COX-2 may be a useful biomarker in RCC.

摘要

目的

环氧化酶（COX）可催化花生四烯酸合成前列腺素，有两种同工型，即COX - 1和COX - 2。有充分证据表明COX - 2在癌症中起重要作用。本研究旨在评估COX - 2表达及其定位在人类肾细胞癌（RCC）发生发展中的临床意义。

方法与结果

通过免疫组化法评估了75例人类RCC组织中COX - 2的表达及定位。在所有RCC病例中均观察到免疫反应性COX - 2蛋白，且COX - 2的表达水平与肿瘤分级和病理分期相关。COX - 2在RCC颗粒细胞亚型中的表达高于透明细胞亚型。免疫电子显微镜显示COX - 2在RCC细胞的核膜、粗面内质网、高尔基体复合体和线粒体膜中表达。