Yoshizawa Katsuhiko, Emoto Yuko, Kinoshita Yuichi, Kimura Ayako, Uehara Norihisa, Yuri Takashi, Shikata Nobuaki, Hamazaki Tomohito, Tsubura Airo
Department of Pathology II, Kansai Medical University, Moriguchi, Osaka 570-8506;
Oncol Lett. 2013 Apr;5(4):1112-1116. doi: 10.3892/ol.2013.1162. Epub 2013 Jan 30.
Arachidonic acid (AA) is naturally found in human breast milk. AA, together with docosahexaenoic acid, is commonly added as a functional food ingredient to commercial infant formula worldwide, in accordance with the international standards of Codex Alimentarius. However, few studies of the possible renal carcinogenic effects of AA supplementation during neonatal life have been performed. The effect of dietary AA supplementation in dams during gestation and lactation was investigated on -methyl--nitrosourea (MNU)-induced preneoplastic lesions in the kidneys of young Lewis rats. Dams were fed a 2.0% AA diet or a basal diet (<0.01% AA). At birth (postnatal day 0), male and female pups received a single intraperitoneal injection of 35 mg/kg MNU or vehicle. Renal morphology was examined after 7, 14, 21, 28 and 60 days. Histopathologically, renal preneoplastic lesions, such as nephroblastomatosis and mesenchymal cell proliferation, were found on day 60 in both the MNU-treated groups. There was no significant difference in lesion incidence of 38% in the basal diet group and 31% in the AA diet group. In conclusion, an AA-rich diet for dams during gestation and lactation does not modify MNU-induced renal preneoplastic lesions in their offspring.
花生四烯酸（AA）天然存在于人乳中。根据食品法典委员会的国际标准，AA与二十二碳六烯酸一起，作为一种功能性食品成分，普遍添加到全球范围内的商业婴儿配方奶粉中。然而，关于新生儿期补充AA可能产生的肾致癌作用的研究很少。研究了孕期和哺乳期母鼠补充膳食AA对年轻Lewis大鼠肾脏中甲基亚硝基脲（MNU）诱导的癌前病变的影响。母鼠喂食含2.0% AA的饮食或基础饮食（<0.01% AA）。出生时（出生后第0天），雄性和雌性幼崽腹腔注射一次35 mg/kg MNU或溶剂。在第7、14、21、28和60天后检查肾脏形态。组织病理学检查发现，在第60天时，两个MNU处理组均出现肾母细胞瘤病和间充质细胞增殖等肾癌前病变。基础饮食组病变发生率为38%，AA饮食组为31%，两组之间无显著差异。总之，孕期和哺乳期母鼠食用富含AA的饮食不会改变其后代中MNU诱导的肾癌前病变。
