Duggan Orla, Hyland Paul, Annett Kathryn, Freeburn Robin, Barnett Christopher, Pawelec Graham, Barnett Yvonne
Cancer and Ageing Research Group, School of Biomedical Sciences, University of Ulster, Coleraine, Northern Ireland BT52 1SA, UK.
Exp Gerontol. 2004 Apr;39(4):525-30. doi: 10.1016/j.exger.2003.12.010.
Oxidative DNA damage has been suggested to contribute to the decline in T cell clone (TCC) function with increased age in vitro. To test this hypothesis the effect of a reduced oxygen tension culture system (6% O(2)) on TCCs was examined. Specifically, the effects of the altered culture conditions on DNA damage levels, in vitro lifespan and proliferative capacity were assessed in five independently derived human CD4+ TCCs. DNA damage levels over the entire lifespan were significantly lowered by reducing oxygen tension. Lifespan (total population doublings (PDs) achieved) and proliferative capacity (PDs/week) were reduced for all clones under reduced oxygen tension when compared to standard culture conditions. This observed tendency warrants further investigation using a greater number of clones from donors of all age groups before definitive conclusions regarding the effect of low oxygen tension on the lifespan and proliferative capacity of TCC can be made. However, these results may suggest that the reduced oxygen tension culture system has interfered with some aspect of T cell biology not yet examined within the remit of this study.
氧化DNA损伤被认为与体外随着年龄增长T细胞克隆（TCC）功能的下降有关。为了验证这一假设，研究了低氧张力培养系统（6% O₂）对TCC的影响。具体而言，在五个独立来源的人类CD4⁺ TCC中评估了改变的培养条件对DNA损伤水平、体外寿命和增殖能力的影响。通过降低氧张力，整个寿命期间的DNA损伤水平显著降低。与标准培养条件相比，在低氧张力下所有克隆的寿命（达到的总群体倍增数（PDs））和增殖能力（每周PDs）均降低。在就低氧张力对TCC寿命和增殖能力的影响得出明确结论之前，这种观察到的趋势需要使用来自所有年龄组供体的更多克隆进行进一步研究。然而，这些结果可能表明，低氧张力培养系统干扰了本研究范围内尚未研究的T细胞生物学的某些方面。
