Balin Arthur K, Fisher Allan J, Anzelone Michael, Leong Ina, Allen R G
Laboratory for Investigative Dermatology, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA.
Exp Cell Res. 2002 Apr 1;274(2):275-87. doi: 10.1006/excr.2002.5485.
It is well known that normal human cells placed in a culture environment exhibit a limited proliferative capacity. The extent to which the culture environment influences proliferative life span is not understood. This study evaluated the effects of the standard procedures used to establish and maintain cultures on the proliferative life spans of different types of human fibroblast cells established from fetal and adult skin and lung. The results of this study demonstrate that procedures to establish cell cultures use only one of several subpopulations of cells present in biopsy pieces and that the culture conditions routinely employed by most laboratories can exert significant effects on proliferative life-span determinations. The maximum proliferative life span differed significantly when obtained by growing the cells in two commonly used commercial media. Proliferative life span was inversely related to ambient oxygen tension and directly related to seeding density in all of the lines examined although lines established from adult skin were much more resistant to toxicity. Enzymatic antioxidant defense levels of fetal skin fibroblasts were much lower than those observed in adult skin fibroblasts, but the effects of oxygen on their life spans were similar. Hyperoxia induced larger increases in glutathione concentration in cell lines with low antioxidant enzyme levels.
众所周知，置于培养环境中的正常人类细胞表现出有限的增殖能力。培养环境对增殖寿命的影响程度尚不清楚。本研究评估了用于建立和维持培养物的标准程序对从胎儿和成人皮肤及肺中分离出的不同类型人类成纤维细胞增殖寿命的影响。本研究结果表明，建立细胞培养物的程序仅使用活检组织中存在的几个细胞亚群之一，并且大多数实验室常规采用的培养条件可对增殖寿命的测定产生显著影响。当在两种常用的商业培养基中培养细胞时，最大增殖寿命存在显著差异。在所检测的所有细胞系中，增殖寿命与环境氧张力呈负相关，与接种密度呈正相关，尽管从成人皮肤建立的细胞系对毒性的耐受性要强得多。胎儿皮肤成纤维细胞的酶促抗氧化防御水平远低于成人皮肤成纤维细胞，但氧对其寿命的影响相似。在抗氧化酶水平较低的细胞系中，高氧诱导谷胱甘肽浓度有更大的增加。
