Sitaru Cassian, Powell Jenny, Messer Gerald, Bröcker Eva-Bettina, Wojnarowska Fenella, Zillikens Detlef
Department of Dermatology, University of Würzburg, Würzburg, Germany.
Obstet Gynecol. 2004 Apr;103(4):757-63. doi: 10.1097/01.AOG.0000115506.76104.ad.
To investigate the sensitivity of immunoblotting and enzyme-linked immunosorbent assay (ELISA) to detect autoantibodies to bullous pemphigoid antigen 180 in patients with pemphigoid gestationis and to correlate autoantibody serum levels with disease activity.
In serum samples obtained from 44 pregnant patients before initiation of therapy and from the same number of healthy blood donors, the autoantibody reactivity was assayed by immunofluorescence microscopy on human skin sections as well as Western blot analysis and 2 different ELISAs by using recombinant forms of the immunodominant domain of BP180. In addition, ELISA reactivity with this autoantigen was assayed in 6 patients during the course of the disease, and its correlation with the clinical disease activity was estimated by applying the Spearman rank correlation test.
By indirect immunofluorescence microscopy, complement-fixing autoantibodies to the dermal-epidermal junction were found in 93% of patients' sera. By immunoblotting and ELISA, autoantibodies to bullous pemphigoid antigen 180 were detected in 93% and 86.3% of pemphigoid gestationis patients, respectively, but in none of the healthy controls. Serum levels of autoantibodies as detected by ELISA paralleled the patients' disease activity.
Our study shows that immunoblotting and ELISA are sensitive tools for the detection of autoantibodies to bullous pemphigoid antigen 180 in patients with pemphigoid gestationis. In addition, the ELISA is useful to monitor autoantibody serum levels.
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研究免疫印迹法和酶联免疫吸附测定（ELISA）检测妊娠类天疱疮患者抗大疱性类天疱疮抗原180自身抗体的敏感性，并将自身抗体血清水平与疾病活动度相关联。
在44例妊娠患者治疗开始前采集的血清样本以及相同数量的健康献血者血清样本中，通过对人皮肤切片进行免疫荧光显微镜检查、蛋白质印迹分析以及使用BP180免疫显性结构域的重组形式进行2种不同的ELISA检测自身抗体反应性。此外，在6例患者疾病过程中检测其与该自身抗原的ELISA反应性，并通过Spearman等级相关检验评估其与临床疾病活动度的相关性。
通过间接免疫荧光显微镜检查，93%的患者血清中发现了针对真皮-表皮交界处的补体结合自身抗体。通过免疫印迹法和ELISA检测，妊娠类天疱疮患者中分别有93%和86.3%检测到抗大疱性类天疱疮抗原180自身抗体，但健康对照者均未检测到。ELISA检测的自身抗体血清水平与患者的疾病活动度平行。
我们的研究表明，免疫印迹法和ELISA是检测妊娠类天疱疮患者抗大疱性类天疱疮抗原180自身抗体的敏感工具。此外，ELISA有助于监测自身抗体血清水平。
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