Matsumura K, Amagai M, Nishikawa T, Hashimoto T
Department of Dermatology, Keio University School of Medicine, Japan.
Arch Dermatol Res. 1996 Aug;288(9):507-9. doi: 10.1007/BF02505245.
A series of previous studies have indicated that the NC16a domain of the 180-kDa bullous pemphigoid antigen (BP180) is the most immunogenic and probably pathogenic region in both bullous pemphigoid and herpes gestationis. To confirm these previous results by a large-scale study, we examined serum from 154 bullous pemphigoid and 43 herpes gestationis patients using an immunoblot technique with the BP180 NC16a domain fusion protein, which had been prepared using cDNA obtained from a human keratinocyte cDNA library by polymerase chain reaction amplification. This fusion protein was recognized by 90% of bullous pemphigoid and 79% of herpes gestationis serum samples, but not by any other disease or normal control serum samples. These results indicate that this system is very sensitive and specific for the detection of anti-BP180 antibodies and should be useful for the diagnosis of various subepidermal bullous diseases.
一系列先前的研究表明，180 kDa大疱性类天疱疮抗原（BP180）的NC16a结构域在大疱性类天疱疮和妊娠疱疹中是最具免疫原性且可能致病的区域。为通过大规模研究证实这些先前的结果，我们使用BP180 NC16a结构域融合蛋白的免疫印迹技术检测了154例大疱性类天疱疮患者和43例妊娠疱疹患者的血清，该融合蛋白是通过聚合酶链反应扩增从人角质形成细胞cDNA文库获得的cDNA制备的。这种融合蛋白在90%的大疱性类天疱疮血清样本和79%的妊娠疱疹血清样本中被识别，但在任何其他疾病或正常对照血清样本中均未被识别。这些结果表明，该系统在检测抗BP180抗体方面非常敏感且特异，应有助于各种表皮下大疱性疾病的诊断。
