El Sanhoty Rafaat, El-Rahman Ahamed Ali Abd, Bögl Klaus Werner
Federal Institute for Risk Assessment (BFR), Thielallee 88-92, D-14195 Berlin, Germany.
Nahrung. 2004 Feb;48(1):13-8. doi: 10.1002/food.200300310.
The aim of this study was to evaluate the composition, nutritional and toxicology safety of GM potato Spunta lines compared to that of conventional potato Spunta. Compositional analyses were conducted to measure the proximate chemical composition with references to 14 components, total solid, protein, lipid, crude fibre, ash, carbohydrate, starch, reducing sugar, nonreducing sugar, sodium, calcium, potassium, phosphorus, and ascorbic acid. Some toxins and anti-nutrients compounds were determined. Feeding study of GM potatoes line (G2 and G3) in rats were done for 30 days. Four groups of albino rats were used for studying the effect and the safety assessment of GM potatoes Spunta G2 and G3. Group (I) was fed on control basal diet, group (II) was fed on control diet plus 30% freeze-dried nongenetically modified potato Spunta, group (III) was fed on control diet plus 30% freeze-dried genetically modified potato Spunta, and group (IV) was fed on control diet plus 30% freeze-dried genetically modified potato Spunta GMO G3. There were no significant differences between GM potatoes G2, G3, and Spunta control potato line in the proximate chemical composition. The levels of glycoalkaloids in transgenic potato tubers and nontransgenic were determined and there were also no significant differences between the GM potatoes and conventional potato line, the levels were in agreement with a safety level recommended by FAO/WHO (200 mg/ kg) for acute toxicity. Protease inhibitor activity and total phenol were estimated and no significant differences between the GM potatoes line and conventional potato Spunta line were found. During the period tested, rats in each group (I, II, III, IV) grew well without marked differences in appearance. No statistical difference were found in food intake, daily body weight gain and feed efficiency. But there is a slightly significant difference in finally body weight between the control group and experimental groups. No significant difference were found in serum biochemical value between each groups, and also between relative organs weight (liver, spleen, heart, kidney, testes). From these results, it can be concluded that the GM potatoes Spunta line (G2 and G3) with Cry V gene are confirmed to have nearly the composition and biochemical characteristics as non-GM potato Spunta.
本研究的目的是评估转基因马铃薯Spunta品系与传统马铃薯Spunta相比的成分、营养和毒理学安全性。进行了成分分析，以测量14种成分的近似化学成分，包括总固体、蛋白质、脂质、粗纤维、灰分、碳水化合物、淀粉、还原糖、非还原糖、钠、钙、钾、磷和抗坏血酸。测定了一些毒素和抗营养化合物。对转基因马铃薯品系（G2和G3）进行了为期30天的大鼠喂养研究。使用四组白化大鼠来研究转基因马铃薯Spunta G2和G3的效果和安全性评估。第一组（I）喂食对照基础日粮，第二组（II）喂食对照日粮加30%冻干非转基因马铃薯Spunta，第三组（III）喂食对照日粮加30%冻干转基因马铃薯Spunta，第四组（IV）喂食对照日粮加30%冻干转基因马铃薯Spunta GMO G3。转基因马铃薯G2、G3与Spunta对照马铃薯品系在近似化学成分上没有显著差异。测定了转基因马铃薯块茎和非转基因马铃薯块茎中的糖苷生物碱水平，转基因马铃薯和传统马铃薯品系之间也没有显著差异，该水平符合粮农组织/世界卫生组织建议的急性毒性安全水平（200毫克/千克）。估计了蛋白酶抑制剂活性和总酚含量，转基因马铃薯品系和传统马铃薯Spunta品系之间没有发现显著差异。在测试期间，每组（I、II、III、IV）的大鼠生长良好，外观上没有明显差异。在食物摄入量、每日体重增加和饲料效率方面没有发现统计学差异。但对照组和实验组之间的最终体重存在轻微显著差异。各组之间以及相对器官重量（肝脏、脾脏、心脏、肾脏、睾丸）之间的血清生化值没有发现显著差异。从这些结果可以得出结论，带有Cry V基因的转基因马铃薯Spunta品系（G2和G3）被证实具有与非转基因马铃薯Spunta几乎相同的成分和生化特性。
